Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA5   Type   Machinery gene
Locus tag   SYNPCCN_RS10465 Genome accession   NC_017052
Coordinates   2218900..2219445 (+) Length   181 a.a.
NCBI ID   WP_223211273.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803 substr. PCC-N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2213900..2224445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYNPCCN_RS10445 - 2213965..2214210 (+) 246 WP_020862237.1 hypothetical protein -
  SYNPCCN_RS10450 (SYNPCCN_2005) - 2214290..2216989 (-) 2700 WP_010873202.1 chloride channel protein -
  SYNPCCN_RS10455 (SYNPCCN_2006) - 2217104..2217607 (+) 504 WP_020862235.1 hypothetical protein -
  SYNPCCN_RS10460 (SYNPCCN_2007) - 2217555..2218775 (+) 1221 WP_010873204.1 DUF58 domain-containing protein -
  SYNPCCN_RS10465 (SYNPCCN_2008) pilA/pilA5 2218900..2219445 (+) 546 WP_223211273.1 type II secretion system protein J Machinery gene
  SYNPCCN_RS16920 (SYNPCCN_2009) - 2219462..2220013 (+) 552 WP_010873206.1 Tfp pilus assembly protein FimT/FimU -
  SYNPCCN_RS10475 (SYNPCCN_2010) - 2220130..2220690 (+) 561 WP_010873207.1 Tfp pilus assembly protein FimT/FimU -
  SYNPCCN_RS16925 (SYNPCCN_2011) - 2220712..2221629 (+) 918 WP_010873208.1 PilW family protein -
  SYNPCCN_RS10480 (SYNPCCN_2012) - 2221687..2222382 (+) 696 WP_010873209.1 alpha/beta fold hydrolase -
  SYNPCCN_RS10485 (SYNPCCN_2013) - 2222603..2222893 (-) 291 WP_223211274.1 hypothetical protein -
  SYNPCCN_RS10490 (SYNPCCN_2014) - 2223092..2223523 (-) 432 WP_010873211.1 hypothetical protein -
  SYNPCCN_RS10495 (SYNPCCN_2015) - 2223580..2224344 (-) 765 WP_010873212.1 IS5 family transposase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 19521.27 Da        Isoelectric Point: 5.2061

>NTDB_id=44492 SYNPCCN_RS10465 WP_223211273.1 2218900..2219445(+) (pilA/pilA5) [Synechocystis sp. PCC 6803 substr. PCC-N]
MFKAIFPRYSVGDRRLLESVRHSAQQGFTMFEVLIALMISFLFLTGTLNAMVMATVMRVKAEREAQAGYWIKEDLEQVRA
LAANFNPATGNGCPTSVGETFRDANNSADADPNNDGLASLPVPANKSIVGVTAAITRTPGVSNNVLTLSYRVRNTNVTDN
DMATLATLYTEVLPAAALACE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=44492 SYNPCCN_RS10465 WP_223211273.1 2218900..2219445(+) (pilA/pilA5) [Synechocystis sp. PCC 6803 substr. PCC-N]
ATGTTTAAGGCTATTTTCCCAAGGTATTCCGTTGGCGATCGCCGTTTGCTAGAGTCAGTCCGCCACTCAGCTCAGCAGGG
TTTCACCATGTTCGAGGTGCTGATTGCCTTGATGATTTCCTTTCTATTTCTGACAGGAACCCTGAATGCCATGGTAATGG
CAACGGTAATGCGGGTTAAAGCGGAAAGAGAGGCCCAAGCTGGCTATTGGATAAAAGAAGATTTAGAGCAAGTTCGGGCA
TTAGCTGCAAACTTCAATCCGGCAACTGGTAATGGATGCCCTACAAGTGTGGGTGAAACTTTTCGAGATGCAAACAATAG
TGCTGACGCCGATCCTAACAATGATGGACTAGCGAGCTTACCAGTTCCTGCCAACAAATCAATTGTCGGTGTAACAGCCG
CTATTACTAGAACACCAGGGGTATCTAATAACGTTTTAACTCTTAGCTATCGTGTCAGGAACACCAATGTCACCGACAAT
GATATGGCTACCCTTGCAACACTTTACACCGAGGTTCTTCCTGCTGCCGCTCTTGCTTGTGAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(24-47)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA5 Synechocystis sp. PCC 6803

100

83.978

0.84

Multiple sequence alignment