Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HN015_RS08110 Genome accession   NZ_CP053421
Coordinates   1642630..1644000 (-) Length   456 a.a.
NCBI ID   WP_002832361.1    Uniprot ID   A0AAN5Y9S9
Organism   Pediococcus acidilactici strain PMC65     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1637630..1649000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HN015_RS08090 (HN015_08125) - 1637772..1638185 (-) 414 WP_004165556.1 Mini-ribonuclease 3 -
  HN015_RS08095 (HN015_08130) cysS 1638178..1639596 (-) 1419 WP_036685851.1 cysteine--tRNA ligase -
  HN015_RS08100 (HN015_08135) gltX 1639753..1641240 (-) 1488 WP_036685848.1 glutamate--tRNA ligase -
  HN015_RS08105 (HN015_08140) - 1641366..1642514 (-) 1149 WP_166481494.1 PIN/TRAM domain-containing protein -
  HN015_RS08110 (HN015_08145) radA 1642630..1644000 (-) 1371 WP_002832361.1 DNA repair protein RadA Machinery gene
  HN015_RS08115 (HN015_08150) - 1644094..1644630 (-) 537 WP_005921602.1 dUTP diphosphatase dut -
  HN015_RS08120 (HN015_08155) - 1644763..1645086 (+) 324 WP_008842291.1 GNAT family N-acetyltransferase -
  HN015_RS08125 (HN015_08160) rpiA 1645097..1645783 (+) 687 WP_008842290.1 ribose-5-phosphate isomerase RpiA -
  HN015_RS08130 (HN015_08165) - 1645829..1647175 (+) 1347 WP_002832366.1 aminopeptidase C -
  HN015_RS08135 (HN015_08170) - 1647388..1648059 (-) 672 WP_024862952.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  HN015_RS08140 (HN015_08175) accA 1648083..1648850 (-) 768 WP_166481495.1 carboxyltransferase subunit alpha -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.79 Da        Isoelectric Point: 7.4153

>NTDB_id=444875 HN015_RS08110 WP_002832361.1 1642630..1644000(-) (radA) [Pediococcus acidilactici strain PMC65]
MAKVKTQFVCNNCGYASPKFLGRCPNCGKWNTLVEERMSDPKAERKSRVSFDGKHTQPQLISDVAMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAETQRKVLYVSGEESASQIKMRAERLKVNSERFYLYPETDMSSVRA
VIEELHPEYVIIDSVQTMQEPDIESAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLKDASGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRAKLTLQNQDAYLKAAGGVKLDEPAIDLALAISIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFKRILIPKNNLKGWQPPEGIEVVGVATIADAIRRAFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=444875 HN015_RS08110 WP_002832361.1 1642630..1644000(-) (radA) [Pediococcus acidilactici strain PMC65]
ATGGCAAAAGTTAAGACCCAGTTTGTATGCAATAATTGCGGATACGCCTCCCCTAAATTTTTAGGACGCTGTCCCAATTG
TGGTAAGTGGAACACTTTAGTTGAAGAACGGATGAGCGATCCAAAGGCTGAACGAAAAAGTCGGGTGAGCTTTGACGGCA
AACATACGCAACCACAGCTGATTTCGGACGTCGCGATGCACGAAGAACCCCGGGTAAAAACCGGGATGGAAGAACTTAAC
CGCGTGCTTGGTGGCGGGGTGGTTGACGGCTCCCTAGTCTTAATTGGTGGGGATCCTGGAATCGGAAAATCAACCCTGTT
GTTACAGCTATCAGGACAGTTGGCAGAGACGCAACGCAAGGTACTTTACGTTTCTGGTGAAGAAAGTGCTTCCCAGATTA
AGATGCGTGCCGAACGATTGAAAGTCAATAGTGAACGCTTCTATTTGTATCCAGAGACCGATATGTCTAGCGTGCGGGCG
GTGATTGAGGAGTTACACCCCGAATACGTGATCATTGATTCGGTTCAGACCATGCAGGAACCGGATATTGAATCGGCGGT
GGGGAGCGTTTCTCAAATTCGAGAAATTACCGCAGAATTAATGCAAATCGCGAAAACCAATAACATTACCATCTTCATCG
TTGGTCACGTGACCAAGGGCGGCGCAATTGCGGGGCCGAAGATTTTGGAACACATGGTGGATACGGTGCTTTACTTTGAA
GGTGATCTGCACCACACTTACCGGATCCTGCGAGCGGTGAAGAACCGGTTTGGTTCCACGAACGAATTAGGAATCTTTGA
GATGCGCGAAGAAGGGTTACGCGAGGTGGCTAACCCGTCAGAAATTTTCTTAGAAGAGCGGCTAAAAGATGCTTCAGGAT
CGGCAATTGTAGTTTCAATGGAAGGAACTCGGCCGATTTTAGTTGAAATACAAGCCTTGATCACGCCCACCATCTTCGGC
AATGCGCAGCGCACGGCGAGCGGGTTGGACCGCAACCGGGTTTCGCTGATCATGGCGGTGCTCGAAAAACGGGCAAAGCT
TACTTTGCAAAACCAAGACGCCTATTTAAAGGCGGCGGGTGGCGTTAAGCTTGACGAACCCGCAATTGACTTAGCGTTGG
CGATTAGTATTGTTTCAAGTTTTAAAAATCAGGGAACCCGGCCGACCGATAGTTTTGTTGGTGAAATCGGCTTAACTGGT
GAAATTCGGCGGGTTAACCGAATTGAACAACGGGTCGCTGAAGCACAAAAGCTTGGTTTTAAGCGGATTTTGATTCCGAA
GAATAATTTGAAGGGGTGGCAGCCCCCCGAAGGAATTGAAGTTGTAGGGGTAGCTACGATTGCCGACGCGATTCGGCGGG
CATTTAATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.033

99.781

0.669
  radA Streptococcus mitis NCTC 12261

66.813

99.781

0.667
  radA Streptococcus pneumoniae Rx1

66.813

99.781

0.667
  radA Streptococcus pneumoniae TIGR4

66.813

99.781

0.667
  radA Streptococcus pneumoniae D39

66.813

99.781

0.667
  radA Streptococcus pneumoniae R6

66.813

99.781

0.667
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654