Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HLV59_RS15975 Genome accession   NZ_CP053391
Coordinates   3417494..3418294 (-) Length   266 a.a.
NCBI ID   WP_214286585.1    Uniprot ID   -
Organism   Acinetobacter lactucae isolate QL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3412494..3423294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLV59_RS15955 - 3413556..3414254 (-) 699 WP_016144303.1 tetratricopeptide repeat protein -
  HLV59_RS15960 hisS 3414283..3415575 (-) 1293 WP_062849551.1 histidine--tRNA ligase -
  HLV59_RS15965 ispG 3415572..3416687 (-) 1116 WP_016144301.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HLV59_RS15970 - 3416709..3417503 (-) 795 WP_031947328.1 DUF4115 domain-containing protein -
  HLV59_RS15975 pilF 3417494..3418294 (-) 801 WP_214286585.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HLV59_RS15980 rlmN 3418309..3419544 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  HLV59_RS15985 ndk 3419668..3420099 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  HLV59_RS15990 iscX 3420213..3420413 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  HLV59_RS15995 - 3420513..3421064 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  HLV59_RS16000 - 3421089..3422378 (-) 1290 WP_171430674.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30001.17 Da        Isoelectric Point: 9.0424

>NTDB_id=444700 HLV59_RS15975 WP_214286585.1 3417494..3418294(-) (pilF) [Acinetobacter lactucae isolate QL-1]
MSIPKLKTVLCMGIAVAFMASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGTQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=444700 HLV59_RS15975 WP_214286585.1 3417494..3418294(-) (pilF) [Acinetobacter lactucae isolate QL-1]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCGGTTGCATTTATGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACAGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAACGCAAGCATTAGTAAACCAA
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.105

100

0.921
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733