Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HNO13_RS01785 Genome accession   NZ_CP053377
Coordinates   361538..361975 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain EN01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 356538..366975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNO13_RS19920 - 357118..357279 (-) 162 Protein_305 hypothetical protein -
  HNO13_RS01750 (HNO13_01755) - 357396..358598 (+) 1203 WP_024084872.1 GTP-binding protein -
  HNO13_RS01755 (HNO13_01760) - 358663..359799 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  HNO13_RS01760 (HNO13_01765) - 359814..360248 (+) 435 WP_021494203.1 RDD family protein -
  HNO13_RS01765 (HNO13_01770) - 360320..360640 (+) 321 WP_015388719.1 YckD family protein -
  HNO13_RS20150 (HNO13_01775) - 360744..360827 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  HNO13_RS01775 (HNO13_01780) - 360859..361077 (+) 219 Protein_311 family 1 glycosylhydrolase -
  HNO13_RS01780 (HNO13_01785) nin/comJ 361118..361516 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  HNO13_RS01785 (HNO13_01790) nucA/comI 361538..361975 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HNO13_RS01790 (HNO13_01795) hxlB 362334..362891 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  HNO13_RS01795 (HNO13_01800) hxlA 362888..363523 (-) 636 WP_024084873.1 3-hexulose-6-phosphate synthase -
  HNO13_RS01800 (HNO13_01805) - 363755..364117 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=444515 HNO13_RS01785 WP_003156588.1 361538..361975(-) (nucA/comI) [Bacillus velezensis strain EN01]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=444515 HNO13_RS01785 WP_003156588.1 361538..361975(-) (nucA/comI) [Bacillus velezensis strain EN01]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641