Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HL660_RS01490 Genome accession   NZ_CP053312
Coordinates   311065..312438 (+) Length   457 a.a.
NCBI ID   WP_407567598.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain WHH2644     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 306065..317438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HL660_RS01460 (HL660_01455) - 306066..306941 (+) 876 WP_407567596.1 prolyl oligopeptidase family serine peptidase -
  HL660_RS01465 (HL660_01460) - 307258..308595 (-) 1338 WP_407567597.1 aminopeptidase C -
  HL660_RS01470 (HL660_01465) - 308659..309273 (-) 615 Protein_272 sugar O-acetyltransferase -
  HL660_RS01475 (HL660_01470) rpiA 309304..309990 (-) 687 WP_021815661.1 ribose-5-phosphate isomerase RpiA -
  HL660_RS01480 (HL660_01475) - 310002..310313 (-) 312 WP_021815662.1 GNAT family N-acetyltransferase -
  HL660_RS01485 (HL660_01480) - 310513..311052 (+) 540 WP_031265151.1 dUTP diphosphatase -
  HL660_RS01490 (HL660_01485) radA 311065..312438 (+) 1374 WP_407567598.1 DNA repair protein RadA Machinery gene
  HL660_RS01495 (HL660_01490) - 312471..313604 (+) 1134 WP_046948740.1 PIN/TRAM domain-containing protein -
  HL660_RS01500 (HL660_01495) gltX 313675..315171 (+) 1497 WP_021815666.1 glutamate--tRNA ligase -
  HL660_RS01505 (HL660_01500) - 315239..315808 (+) 570 WP_046948741.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49352.75 Da        Isoelectric Point: 8.9999

>NTDB_id=444010 HL660_RS01490 WP_407567598.1 311065..312438(+) (radA) [Limosilactobacillus fermentum strain WHH2644]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGALAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTNAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=444010 HL660_RS01490 WP_407567598.1 311065..312438(+) (radA) [Limosilactobacillus fermentum strain WHH2644]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGCCTTAGCCGGTCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGTGGTTTGACCGAGGTCGCAAATCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCAACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.212

99.125

0.676
  radA Streptococcus mitis NCTC 12261

67.991

99.125

0.674
  radA Streptococcus pneumoniae Rx1

67.991

99.125

0.674
  radA Streptococcus pneumoniae D39

67.991

99.125

0.674
  radA Streptococcus pneumoniae R6

67.991

99.125

0.674
  radA Streptococcus pneumoniae TIGR4

67.991

99.125

0.674
  radA Bacillus subtilis subsp. subtilis str. 168

63.457

100

0.635