Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K562_RS04150 Genome accession   NZ_CP053300
Coordinates   873902..875407 (-) Length   501 a.a.
NCBI ID   WP_006483132.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain K56-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 868902..880407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K562_RS04125 (K562_10837) - 869933..870568 (-) 636 WP_006483137.1 LON peptidase substrate-binding domain-containing protein -
  K562_RS04130 (K562_10838) rapZ 870595..871503 (-) 909 WP_006483144.1 RNase adapter RapZ -
  K562_RS04135 (K562_10839) hprK 871578..872546 (-) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  K562_RS04140 (K562_10840) - 872676..873188 (-) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  K562_RS04145 (K562_10841) hpf 873384..873743 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K562_RS04150 (K562_10842) rpoN/rpoN1 873902..875407 (-) 1506 WP_006483132.1 RNA polymerase factor sigma-54 Machinery gene
  K562_RS04155 (K562_10843) lptB 875580..876356 (-) 777 WP_006483143.1 LPS export ABC transporter ATP-binding protein -
  K562_RS04160 (K562_10844) lptA 876353..877018 (-) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  K562_RS04165 (K562_10845) lptC 877039..877653 (-) 615 WP_006483145.1 LPS export ABC transporter periplasmic protein LptC -
  K562_RS04170 (K562_10846) - 877657..878193 (-) 537 WP_006483148.1 HAD family hydrolase -
  K562_RS04175 (K562_10847) kdsD 878193..879176 (-) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54735.87 Da        Isoelectric Point: 4.8740

>NTDB_id=443993 K562_RS04150 WP_006483132.1 873902..875407(-) (rpoN/rpoN1) [Burkholderia cenocepacia strain K56-2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSPSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=443993 K562_RS04150 WP_006483132.1 873902..875407(-) (rpoN/rpoN1) [Burkholderia cenocepacia strain K56-2]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCGCCGCG
ACCAACGGCAGCAGCCCGTCCAGCGATCGCGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTCAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGTGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTTGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689