Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HLY09_RS13410 Genome accession   NZ_CP053229
Coordinates   2869684..2871072 (-) Length   462 a.a.
NCBI ID   WP_002574319.1    Uniprot ID   A0A6M5G7E6
Organism   Enterocloster bolteae strain CBBP-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2864684..2876072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLY09_RS13395 (HLY09_13450) - 2866607..2867320 (+) 714 WP_002574321.1 FadR/GntR family transcriptional regulator -
  HLY09_RS13400 (HLY09_13455) - 2867400..2869169 (+) 1770 WP_002591583.1 FAD-binding protein -
  HLY09_RS13410 (HLY09_13465) radA 2869684..2871072 (-) 1389 WP_002574319.1 DNA repair protein RadA Machinery gene
  HLY09_RS13415 (HLY09_13470) - 2871161..2871577 (-) 417 WP_002567200.1 hypothetical protein -
  HLY09_RS13420 (HLY09_13475) - 2871696..2874305 (-) 2610 WP_002591582.1 ATP-dependent Clp protease ATP-binding subunit -
  HLY09_RS13425 (HLY09_13480) - 2874359..2875393 (-) 1035 WP_002574317.1 ATP--guanido phosphotransferase -
  HLY09_RS13430 (HLY09_13485) - 2875386..2876003 (-) 618 WP_002574316.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50006.60 Da        Isoelectric Point: 5.6461

>NTDB_id=443638 HLY09_RS13410 WP_002574319.1 2869684..2871072(-) (radA) [Enterocloster bolteae strain CBBP-2]
MAKARTTAFFCKECGYESSKWMGQCPACKAWNSMVEEPVSKPAGSQAGLGRLASGSQKSPVPAARPSLLSEIDIEEQDRI
STGFGELDRVLGDGIVAGSLVLVGGDPGIGKSTLLLQVCRNLAGAGQRVLYISGEESLKQIKMRANRIGPVTGELKFLCE
TSLEHIERAIDAENPQIAVIDSIQTMYREEISSAPGSVSQVRESTGILMQIAKSRGIAIFVVGHVTKEGVVAGPRVLEHM
VDTVLYFEGDRNASYRILRSVKNRFGSTNEIGVFEMQEQGLAEVENPSEYMLDGRPEEASGAVVSCSFEGTRPILLEVQA
LVTETNFGMPRRTAAGTDYNRVNLLMAVLEKRCRYEMSRLDAYVNIAGGMKMNEPALDLAIIMALMSSYKDRPVDPKMLI
FGEVGLSGEVRAVSQASQRVNEAAKLGFTACVLPRVCADKMKPVEGIRLIGVSNVREAIGIM

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=443638 HLY09_RS13410 WP_002574319.1 2869684..2871072(-) (radA) [Enterocloster bolteae strain CBBP-2]
ATGGCAAAAGCGAGAACCACCGCATTTTTCTGTAAGGAGTGCGGATATGAATCATCCAAATGGATGGGGCAGTGTCCTGC
CTGCAAGGCCTGGAATTCCATGGTGGAGGAACCGGTGTCAAAGCCGGCAGGCAGTCAGGCCGGGTTAGGGCGCCTGGCTT
CGGGAAGCCAGAAGTCCCCGGTTCCGGCAGCCAGACCATCTCTGCTGTCAGAGATTGACATAGAGGAGCAGGACCGTATA
TCCACGGGCTTTGGGGAACTGGACCGGGTGCTGGGAGACGGAATCGTGGCAGGCTCCCTGGTGCTGGTGGGCGGAGACCC
GGGTATCGGAAAATCCACCCTTCTTCTTCAGGTATGCCGGAATCTGGCGGGCGCAGGCCAGCGGGTCCTGTACATATCAG
GGGAGGAATCCTTAAAACAGATTAAGATGAGGGCAAACCGCATCGGTCCTGTAACCGGAGAGCTGAAATTCCTGTGTGAG
ACAAGTCTGGAGCATATTGAGAGGGCCATTGACGCTGAGAATCCCCAGATTGCAGTTATTGATTCCATACAGACCATGTA
CAGGGAGGAGATATCCTCTGCTCCCGGAAGCGTCAGCCAGGTAAGGGAGTCCACGGGAATCCTGATGCAGATAGCAAAGA
GCCGGGGAATTGCCATTTTTGTGGTGGGGCATGTGACAAAGGAAGGCGTGGTGGCAGGCCCCAGGGTACTGGAACATATG
GTGGATACGGTACTCTATTTTGAGGGAGACAGGAATGCGTCCTACCGCATTCTGCGCAGTGTGAAAAACCGGTTTGGATC
TACCAATGAGATAGGTGTATTTGAGATGCAGGAACAGGGGCTGGCAGAGGTGGAGAATCCCTCTGAATACATGCTGGACG
GCCGTCCGGAGGAAGCCTCCGGCGCAGTGGTATCCTGTTCCTTTGAGGGTACCAGGCCCATTCTTTTAGAGGTACAGGCC
CTGGTGACGGAAACGAATTTCGGTATGCCCAGGCGGACGGCGGCCGGTACGGATTACAACCGTGTGAACCTGCTGATGGC
AGTTCTGGAGAAGCGCTGCCGCTATGAGATGTCGCGGCTGGATGCCTATGTGAATATAGCGGGAGGCATGAAGATGAACG
AGCCCGCCCTGGATCTGGCTATTATCATGGCCCTTATGTCCAGCTACAAAGACCGGCCCGTGGATCCGAAAATGCTCATA
TTCGGGGAGGTGGGCCTGTCAGGAGAGGTCAGGGCCGTATCCCAGGCCAGCCAGCGTGTGAATGAGGCTGCCAAGCTGGG
CTTTACAGCCTGTGTGCTGCCAAGAGTATGCGCGGATAAGATGAAGCCTGTGGAGGGCATACGTTTGATTGGGGTGTCCA
ATGTGCGGGAGGCCATTGGAATCATGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(86-229)

Subunit ChlI of Mg-chelatase (ChlI)

(351-435)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M5G7E6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.739

99.567

0.565
  radA Streptococcus mitis NCTC 12261

51.634

99.351

0.513
  radA Streptococcus mitis SK321

51.634

99.351

0.513
  radA Streptococcus pneumoniae Rx1

51.416

99.351

0.511
  radA Streptococcus pneumoniae D39

51.416

99.351

0.511
  radA Streptococcus pneumoniae R6

51.416

99.351

0.511
  radA Streptococcus pneumoniae TIGR4

51.416

99.351

0.511