Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EGM87_RS00320 Genome accession   NZ_CP053222
Coordinates   74754..76121 (+) Length   455 a.a.
NCBI ID   WP_044661660.1    Uniprot ID   -
Organism   Sphingobium sp. RSMS     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 69754..81121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM87_RS00295 (EGM87_00295) - 70108..70533 (-) 426 WP_044663047.1 metallopeptidase family protein -
  EGM87_RS00300 (EGM87_00300) - 70620..70907 (+) 288 WP_137709343.1 4a-hydroxytetrahydrobiopterin dehydratase -
  EGM87_RS00305 (EGM87_00305) - 70912..71520 (-) 609 WP_061940249.1 hypothetical protein -
  EGM87_RS00310 (EGM87_00310) - 71517..73850 (-) 2334 WP_137709342.1 patatin-like protein -
  EGM87_RS00315 (EGM87_00315) - 73966..74664 (-) 699 WP_171009986.1 endonuclease/exonuclease/phosphatase family protein -
  EGM87_RS00320 (EGM87_00320) radA 74754..76121 (+) 1368 WP_044661660.1 DNA repair protein RadA Machinery gene
  EGM87_RS00325 (EGM87_00325) - 76133..76648 (+) 516 WP_044661661.1 CvpA family protein -
  EGM87_RS00330 (EGM87_00330) - 76938..77120 (+) 183 WP_025551144.1 general stress protein CsbD -
  EGM87_RS00335 (EGM87_00335) - 77240..78562 (+) 1323 WP_137709340.1 aminopeptidase P family protein -
  EGM87_RS00340 (EGM87_00340) - 78958..80331 (-) 1374 WP_137709339.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47725.58 Da        Isoelectric Point: 6.5868

>NTDB_id=443607 EGM87_RS00320 WP_044661660.1 74754..76121(+) (radA) [Sphingobium sp. RSMS]
MAKAKRKFVCQQCGTVSGRWQGQCDDCGEWNSIVEEAAETVFSARHDLHSGGRALTLVGLDDKVELPPRTTTGIAEFDRA
LGGGIVSGSATLIGGDPGIGKSTLLLQAAARIASAGHSVAYISGEEASDQVRLRAQRLGLGNAPVQLASATSVRDILTTL
GEGAPPALLIIDSIQTMHSDLIEGAPGTVSQVRASSQELIRFAKQRGTALILVGHVTKDGSIAGPRVLEHMVDTVLSFEG
ERSHQYRILRAIKNRFGGTDEIGVFAMVAEGLEEVANPSALFLTHRDENVTGATVFPALEGTRPVLVEIQALVVRLSSGA
TPRRAVVGWDSGRLAMVLAVLEARCGLSFSTCEVYLNVAGGYRLSDPAADLAVAAALMSALSERPVPADVVLFGEIALSG
EIRPVAHAPLRLREAAKLGFNRAFVPAAVADGVKGISVSGYRALAQLVDQMLGRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=443607 EGM87_RS00320 WP_044661660.1 74754..76121(+) (radA) [Sphingobium sp. RSMS]
ATGGCCAAGGCAAAGCGAAAATTCGTCTGTCAGCAATGCGGCACCGTTTCGGGCCGGTGGCAGGGGCAGTGCGACGATTG
CGGCGAATGGAACAGCATTGTCGAGGAAGCGGCCGAGACGGTCTTTTCCGCCCGCCACGACCTGCATAGCGGCGGCAGGG
CGCTGACGCTGGTGGGGCTGGACGACAAGGTCGAACTGCCGCCCCGCACCACCACCGGCATTGCGGAGTTCGATCGGGCG
CTGGGCGGCGGCATCGTGTCCGGTTCCGCCACGCTGATCGGCGGCGATCCGGGCATAGGCAAGTCGACCCTGCTGTTGCA
GGCGGCCGCGCGCATTGCTTCGGCCGGGCACAGCGTCGCCTATATCAGCGGCGAGGAAGCGTCGGATCAGGTGCGCCTGC
GCGCGCAGCGGCTGGGGCTTGGCAATGCGCCCGTGCAGCTTGCCAGCGCCACTTCCGTGCGCGACATACTGACGACGCTG
GGGGAGGGCGCGCCGCCCGCGCTGCTGATCATCGATTCGATCCAGACGATGCACAGCGACCTGATCGAGGGCGCGCCCGG
CACGGTGAGCCAGGTGCGCGCGTCCAGCCAGGAACTGATCCGCTTCGCCAAGCAGCGCGGCACCGCCCTGATCCTTGTCG
GTCATGTGACCAAGGACGGCAGCATCGCGGGCCCCCGCGTGCTGGAGCATATGGTCGATACGGTGCTGAGCTTCGAGGGA
GAGCGCAGCCACCAATATCGCATCCTGCGCGCGATCAAGAACCGCTTCGGCGGCACGGACGAGATCGGCGTGTTCGCGAT
GGTGGCCGAGGGGCTGGAGGAGGTCGCCAATCCGTCCGCCCTGTTCCTGACCCATCGCGACGAGAATGTGACCGGCGCCA
CCGTCTTTCCGGCGCTGGAAGGGACGCGCCCGGTGCTGGTGGAGATCCAGGCGCTGGTCGTCCGCCTTTCGAGCGGGGCC
ACGCCCCGCCGGGCTGTCGTGGGTTGGGACAGCGGGCGGCTGGCCATGGTGCTGGCCGTGCTGGAGGCGCGCTGCGGCCT
CAGCTTTTCGACCTGCGAAGTCTATCTGAACGTCGCGGGCGGCTATCGCCTGTCGGACCCGGCGGCCGACCTGGCTGTCG
CGGCCGCGCTGATGTCGGCGCTGTCGGAACGGCCGGTGCCCGCCGATGTCGTCCTGTTCGGGGAAATCGCCTTGTCCGGC
GAAATCCGCCCGGTGGCCCATGCCCCGTTGCGGTTGCGCGAGGCGGCCAAGCTCGGTTTCAACCGCGCTTTCGTTCCCGC
CGCCGTGGCCGATGGGGTCAAGGGCATTTCGGTCAGCGGTTATCGCGCCCTTGCGCAACTTGTTGACCAGATGCTCGGCC
GCGGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(82-220)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.052

100

0.488
  radA Streptococcus mitis NCTC 12261

46.171

100

0.464
  radA Streptococcus pneumoniae Rx1

45.952

100

0.462
  radA Streptococcus pneumoniae D39

45.952

100

0.462
  radA Streptococcus pneumoniae R6

45.952

100

0.462
  radA Streptococcus pneumoniae TIGR4

45.952

100

0.462
  radA Streptococcus mitis SK321

47.356

95.604

0.453