Detailed information NTG0443542

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HLG75_RS14590
• Genome accession: NZ_CP053215
• Coordinates: 3003506..3004624 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain DT0544C
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2998506..3009624

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HLG75_RS14580 (HLG75_14600)	-	3001622..3002314 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
HLG75_RS14585 (HLG75_14605)	pilT	3002441..3003478 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HLG75_RS14590 (HLG75_14610)	pilU	3003506..3004624 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HLG75_RS14595 (HLG75_14615)	fur	3004694..3005131 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HLG75_RS14600 (HLG75_14620)	-	3005243..3005641 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HLG75_RS14605 (HLG75_14625)	-	3005696..3006019 (-)	324	WP_000442590.1	RnfH family protein	-
HLG75_RS14610 (HLG75_14630)	-	3006019..3007086 (-)	1068	WP_004748097.1	hypothetical protein	-
HLG75_RS14615 (HLG75_14635)	-	3007239..3007694 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HLG75_RS14620 (HLG75_14640)	-	3007947..3008795 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=443542 HLG75_RS14590 WP_000347034.1 3003506..3004624(+) (pilU) [Acinetobacter baumannii strain DT0544C]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=443542 HLG75_RS14590 WP_000347034.1 3003506..3004624(+) (pilU) [Acinetobacter baumannii strain DT0544C]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39