Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   SYNGTI_RS02325 Genome accession   NC_017038
Coordinates   492232..494841 (+) Length   869 a.a.
NCBI ID   WP_010871656.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803 substr. GT-I     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487232..499841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYNGTI_RS02305 (SYNGTI_0448) - 487569..489479 (+) 1911 WP_014407070.1 long-chain fatty acid--CoA ligase -
  SYNGTI_RS02310 (SYNGTI_0449) - 489824..490990 (+) 1167 WP_010871653.1 response regulator -
  SYNGTI_RS02315 (SYNGTI_0450) pilH 491186..491551 (+) 366 WP_014407071.1 response regulator transcription factor Machinery gene
  SYNGTI_RS02320 (SYNGTI_0451) - 491598..492161 (+) 564 WP_010871655.1 chemotaxis protein CheW -
  SYNGTI_RS02325 (SYNGTI_0452) pilJ 492232..494841 (+) 2610 WP_010871656.1 methyl-accepting chemotaxis protein Machinery gene
  SYNGTI_RS02330 (SYNGTI_0453) - 494930..495721 (+) 792 WP_010871657.1 ABC transporter permease -
  SYNGTI_RS02335 (SYNGTI_0454) - 495851..496108 (+) 258 WP_041425909.1 TatA/E family twin arginine-targeting protein translocase -
  SYNGTI_RS02340 (SYNGTI_0455) - 496552..497946 (+) 1395 WP_010871659.1 sensor domain-containing diguanylate cyclase -
  SYNGTI_RS17270 - 498008..498175 (+) 168 WP_158299069.1 hypothetical protein -

Sequence

Protein


Download         Length: 869 a.a.        Molecular weight: 93209.76 Da        Isoelectric Point: 4.0166

>NTDB_id=44332 SYNGTI_RS02325 WP_010871656.1 492232..494841(+) (pilJ) [Synechocystis sp. PCC 6803 substr. GT-I]
MATETNFSQLYGLAYAAYGQGNYQEASSHIEQLAADFPEDPNVLLLRGHIYVGLEQYALAHQAYQGVIRFSDRQDLIDCA
NQALGQIQEEEPRGNGAKDSLDQDWQSDIDAIDNSMAWETGVFTEEDFGEPTLGRAPSPTNPGMDHPFGRSSASFGTSGA
RSYEIENLDWSPEAFTDDLDSDDEQTMMPQTGESTFVVPPAFGEGIADFADLAADVSSAGIGGTDGAEYPPYQTWDGGDW
SEALGLDDPEALAQAPTPTSGDYGAGNNLPSFDAFEEGVEDELSDFNLTDIAPELPDSSLFSQATNLDIGAVGRIDDTQG
GDTVVHGGNAAPPGMATWLKPDGAATGDLSSATSPFNDRPTSEVDAGFLGWFTNASLFRKQIYMAIASGVFSGFAVLVLG
SIVGLGGTPKDVPAPSGETTTEAPAEGAPAEGQAPSQTPEEEPGKPSLLNLAFLTAIATAIGVFLINRLLMQQIKSIIDD
LQNQFESIYEGNFNVRAKVRSEDELGALTQRFNYISQVILTATSEAQERAATTEKVREKLQRQVIRLLDDVEGASRGDLT
VQAEVTADELGAVADAFNLTIQNLREIVLQVKNAAKQVNNNCKDSESFARNNSSDALRMAEELAVTLNSVQLMTESIERV
AENAREAEEVAHTSSLTALKGGEAVERTVGGILQIRETVSETARKVKRLAEASQEISKIVAVISQIASRTNLLALNASIQ
AARAGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTDKSEQSKRALEDIIEVSNR
INTLVRSITADTVKQQENSGAVSNVMQSIELTAQETSQESQRVAGSLQTLVSISRDLLTSVERFRVESR

Nucleotide


Download         Length: 2610 bp        

>NTDB_id=44332 SYNGTI_RS02325 WP_010871656.1 492232..494841(+) (pilJ) [Synechocystis sp. PCC 6803 substr. GT-I]
ATGGCAACAGAAACCAATTTTTCGCAGCTTTATGGGTTGGCCTACGCCGCCTATGGACAGGGGAATTATCAAGAAGCTTC
TTCCCATATTGAACAATTGGCCGCTGATTTTCCCGAAGATCCTAATGTATTGTTGCTCCGGGGGCATATCTATGTCGGCC
TGGAACAATACGCCCTAGCCCACCAGGCTTATCAGGGGGTAATTCGGTTTTCCGATCGCCAAGACTTAATTGACTGCGCT
AACCAGGCCCTAGGGCAAATCCAAGAAGAGGAACCAAGGGGCAACGGAGCCAAAGATTCCCTTGACCAGGATTGGCAAAG
TGACATCGATGCCATTGACAATTCCATGGCCTGGGAAACAGGAGTGTTCACCGAAGAGGATTTCGGCGAGCCCACTTTGG
GCCGGGCCCCCAGCCCCACTAATCCGGGCATGGACCATCCCTTTGGCCGCAGCTCTGCTTCCTTTGGTACCAGTGGGGCC
AGGAGTTACGAAATTGAAAACCTAGATTGGAGTCCGGAAGCTTTCACCGATGACCTGGACTCCGATGATGAACAGACCAT
GATGCCCCAAACTGGCGAGTCTACTTTTGTGGTGCCACCGGCCTTTGGTGAAGGGATAGCGGATTTTGCCGATTTAGCGG
CGGATGTCTCCTCAGCCGGCATTGGCGGGACAGATGGTGCCGAATATCCCCCATATCAAACTTGGGACGGGGGCGACTGG
AGTGAAGCTCTCGGTTTGGATGATCCTGAAGCCTTGGCTCAGGCCCCTACCCCCACCTCTGGCGATTACGGCGCTGGCAA
TAACCTACCGAGTTTTGATGCGTTTGAGGAAGGGGTGGAGGATGAGTTGTCCGATTTTAACCTGACGGATATTGCCCCAG
AGTTGCCCGATTCTTCCCTCTTTAGCCAAGCCACTAATTTGGACATAGGTGCGGTGGGTCGCATCGATGACACCCAGGGC
GGTGATACGGTGGTCCATGGAGGTAATGCAGCGCCACCGGGCATGGCCACTTGGTTGAAACCGGATGGGGCCGCCACCGG
GGATTTATCCAGCGCCACTAGTCCCTTTAATGACCGGCCCACCAGTGAGGTAGATGCGGGTTTTCTCGGTTGGTTCACCA
ATGCTTCCCTGTTTAGAAAGCAGATTTACATGGCGATCGCCTCCGGTGTGTTTTCCGGGTTCGCGGTGTTGGTATTGGGA
AGCATAGTGGGTTTAGGGGGAACTCCCAAAGACGTACCTGCTCCTTCAGGGGAAACCACCACCGAAGCTCCAGCAGAAGG
TGCCCCCGCAGAGGGCCAGGCCCCTTCCCAGACCCCAGAGGAAGAACCGGGCAAACCATCCCTCCTCAACCTCGCCTTCC
TCACAGCCATAGCCACGGCGATCGGAGTCTTTCTGATCAATCGATTGCTGATGCAACAGATTAAGAGTATTATCGACGAC
CTGCAAAATCAGTTTGAATCCATCTATGAGGGCAATTTCAATGTCCGGGCCAAGGTGCGATCGGAGGATGAATTGGGGGC
TTTGACCCAACGGTTTAACTATATTTCCCAAGTTATTCTCACGGCCACTAGTGAAGCCCAGGAACGGGCTGCTACCACCG
AAAAAGTGCGGGAAAAATTACAGCGCCAAGTAATTCGGCTCCTGGACGATGTAGAGGGAGCTTCCCGGGGAGATTTAACC
GTACAAGCGGAAGTAACGGCGGATGAGCTGGGAGCGGTGGCCGATGCCTTTAACCTAACTATCCAAAACCTGCGGGAGAT
TGTGTTACAAGTTAAAAATGCCGCCAAGCAGGTGAATAACAACTGTAAAGACAGTGAGTCCTTTGCCCGCAATAACTCCA
GCGATGCGTTGCGAATGGCGGAGGAATTGGCCGTCACCCTCAACTCTGTTCAGTTAATGACGGAATCCATTGAACGGGTA
GCAGAAAACGCCCGGGAAGCGGAGGAAGTGGCCCATACTTCATCTTTAACTGCCCTCAAAGGTGGGGAAGCGGTGGAAAG
AACGGTGGGGGGCATTCTGCAAATTCGGGAAACGGTGTCGGAAACTGCCCGCAAGGTAAAACGGTTAGCGGAAGCATCCC
AGGAAATTTCCAAAATTGTGGCGGTTATTTCCCAAATTGCCTCCCGGACTAACCTGTTAGCTTTGAACGCCTCCATCCAG
GCGGCCCGGGCAGGGGAAGCAGGGCGGGGCTTTGCCATCGTTGCAGACGAGGTGCGACAGTTGGCGGATCGTTCTGCTAA
GTCGTTGAAGGAAATTGAGCAAATCGTTTTGCAAATTCAGAGTGAAACCGGCTCCGTAATGACCGCCATGGAAGAAGGTA
TCCAACAGGTAATCGACGTAACTGACAAGTCGGAACAGTCGAAACGGGCCCTGGAGGATATTATTGAGGTGTCTAATCGG
ATTAACACTTTGGTGCGTTCCATTACTGCTGATACCGTCAAGCAGCAGGAGAACTCCGGTGCAGTGTCCAACGTCATGCA
GTCCATTGAGCTGACGGCCCAGGAAACTTCCCAGGAATCTCAACGGGTAGCAGGATCTTTGCAAACCCTGGTCTCCATTT
CCCGCGACCTGTTAACTTCGGTGGAACGGTTCCGGGTGGAAAGTCGCTAG

Domains

Predicted by InterproScan.

HAMP domain (HAMP)

(555-586)

HAMP domain (HAMP)

(473-514)

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(649-830)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

100

100

1

Multiple sequence alignment