Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HLJ12_RS00110 Genome accession   NZ_CP053180
Coordinates   22741..24111 (+) Length   456 a.a.
NCBI ID   WP_005226394.1    Uniprot ID   A0A242CQN0
Organism   Enterococcus casseliflavus strain SP11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17741..29111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLJ12_RS00080 (HLJ12_00080) - 18208..19026 (+) 819 WP_194185236.1 ZIP family metal transporter -
  HLJ12_RS00085 (HLJ12_00085) - 19082..19285 (-) 204 WP_010748476.1 hypothetical protein -
  HLJ12_RS00090 (HLJ12_00090) - 19436..19837 (+) 402 WP_194185235.1 DUF4809 family protein -
  HLJ12_RS00095 (HLJ12_00095) - 19941..21185 (-) 1245 WP_005226389.1 glutamate-5-semialdehyde dehydrogenase -
  HLJ12_RS00100 (HLJ12_00100) proB 21182..21997 (-) 816 WP_005226390.1 glutamate 5-kinase -
  HLJ12_RS00105 (HLJ12_00105) - 22187..22696 (+) 510 WP_010748479.1 hypothetical protein -
  HLJ12_RS00110 (HLJ12_00110) radA 22741..24111 (+) 1371 WP_005226394.1 DNA repair protein RadA Machinery gene
  HLJ12_RS00115 (HLJ12_00115) - 24280..25419 (+) 1140 WP_005226396.1 PIN/TRAM domain-containing protein -
  HLJ12_RS00120 (HLJ12_00120) gltX 25589..27046 (+) 1458 WP_010748481.1 glutamate--tRNA ligase -
  HLJ12_RS00125 (HLJ12_00125) epsC 27307..27843 (+) 537 WP_034695645.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49604.28 Da        Isoelectric Point: 8.3034

>NTDB_id=443197 HLJ12_RS00110 WP_005226394.1 22741..24111(+) (radA) [Enterococcus casseliflavus strain SP11]
MAKKAKSQFVCQNCGYSSPKYLGKCPNCGSWNSMVEEVIQETADRRVRTTLTGQKMQPTKIADVVPKKEPRVQTKLAELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAAIGGTVLYVSGEESAEQIKMRAQRLGAIDTEFYLYGETDMSEISK
AIENIRPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHSFRILRAVKNRFGSTNEIGIFEMEEGGLKEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTSPTECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFTKVYLPKNNLTGWQPPKGIEVEGVSTLAETLRKVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=443197 HLJ12_RS00110 WP_005226394.1 22741..24111(+) (radA) [Enterococcus casseliflavus strain SP11]
ATGGCAAAAAAAGCAAAAAGTCAATTTGTCTGTCAAAATTGTGGGTATAGTTCCCCTAAATATTTAGGGAAATGCCCCAA
TTGCGGCAGCTGGAATTCAATGGTGGAAGAAGTGATCCAAGAAACCGCCGATCGTCGAGTACGTACGACATTGACGGGGC
AAAAGATGCAGCCGACGAAAATCGCCGATGTCGTACCGAAAAAAGAACCGCGAGTCCAAACGAAACTTGCTGAGTTGAAT
CGCGTCTTAGGCGGCGGCGTGGTCCCAGGCTCCTTGATTTTGATCGGTGGGGATCCAGGGATCGGAAAATCGACGCTGCT
CTTGCAAGTTTCCCAGCAATTAGCAGCGATCGGCGGGACGGTCTTGTATGTGTCAGGAGAAGAAAGTGCCGAACAAATCA
AAATGCGGGCGCAGCGCTTAGGCGCCATCGACACAGAATTTTATCTCTATGGGGAAACGGACATGTCAGAGATCAGTAAA
GCGATCGAAAACATCCGCCCAGATTATGTGATCATCGATTCGATCCAAACCATGACCCAACCGGATATCACCAGCGTGGC
AGGCAGTGTCAGCCAAGTGCGAGAAACGACCGCAGAATTGCTGAAAATCGCCAAAACCAATGGCATCGCCATCTTTATCG
TTGGCCACGTGACGAAGGAAGGCTCGATCGCAGGACCGCGGATGCTGGAGCATATGGTGGATACCGTCTTGTATTTCGAA
GGCGAAAAGCACCACAGTTTTCGAATTTTGCGGGCCGTAAAAAACCGCTTTGGGTCCACCAATGAGATCGGGATTTTTGA
AATGGAAGAAGGCGGGTTGAAAGAAGTCGCCAATCCGTCACAAGTCTTTTTGGAAGAACGCTTAGAAGGCGCAACTGGAT
CGGCGATCGTAGTGGCAATGGAAGGCAGTCGGCCAATCTTGGTGGAAATCCAAGCACTGGTCACGCCAACGATGTTCGGC
AATGCCAAACGCACGACGACGGGGCTAGACTTCAACCGCGTCTCGCTGATCATGGCGGTTTTAGAAAAACGGGCAGGCTT
GCTGCTGCAAAATCAAGATGCCTACTTAAAAGCAGCCGGCGGGGTCAAATTAAATGAACCCGCGATCGATCTAGCGATCG
CTGTTAGTATCGCGTCTAGCTACAAAGAAAAAGGCACCAGCCCGACGGAGTGTTTTATCGGTGAAATTGGCTTGACGGGT
GAGATTCGCCGAGTCAACGCGATCGAGCAACGGGTCAAAGAGGTGCAAAAACTAGGTTTTACGAAAGTTTATTTGCCGAA
AAATAATCTGACTGGCTGGCAGCCGCCCAAAGGCATCGAAGTCGAAGGCGTGTCTACCTTAGCCGAAACCTTGCGGAAAG
TCTTCCGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A242CQN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.505

99.781

0.743
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664