Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   HIR77_RS20835 Genome accession   NZ_CP053102
Coordinates   3946472..3947617 (+) Length   381 a.a.
NCBI ID   WP_003227549.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3941472..3952617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR77_RS20815 (HIR77_20855) albF 3941488..3942768 (+) 1281 WP_003242974.1 pitrilysin family protein -
  HIR77_RS20820 (HIR77_20860) albG 3942765..3943466 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  HIR77_RS20825 (HIR77_20865) ywhL 3943472..3944848 (-) 1377 WP_003244415.1 YncE family protein -
  HIR77_RS20830 (HIR77_20870) ywhK 3944887..3946242 (-) 1356 WP_003227552.1 YncE family protein -
  HIR77_RS22960 (HIR77_20875) - 3946239..3946469 (-) 231 WP_009968328.1 hypothetical protein -
  HIR77_RS20835 (HIR77_20880) rapF 3946472..3947617 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  HIR77_RS20840 (HIR77_20885) phrF 3947601..3947720 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  HIR77_RS20845 (HIR77_20890) ywhH 3947819..3948292 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  HIR77_RS20850 (HIR77_20895) speB 3948324..3949196 (-) 873 WP_003227545.1 agmatinase -
  HIR77_RS20855 (HIR77_20900) speE 3949257..3950087 (-) 831 WP_003227543.1 spermidine synthase -
  HIR77_RS20860 (HIR77_20905) pbpG 3950289..3952364 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45609.09 Da        Isoelectric Point: 5.1503

>NTDB_id=443136 HIR77_RS20835 WP_003227549.1 3946472..3947617(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=443136 HIR77_RS20835 WP_003227549.1 3946472..3947617(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_12)

(223-290)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572