Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HIR77_RS14115 Genome accession   NZ_CP053102
Coordinates   2670989..2672026 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2665989..2677026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR77_RS14065 (HIR77_14085) - 2666475..2666964 (-) 490 Protein_2740 GNAT family N-acetyltransferase -
  HIR77_RS14070 (HIR77_14090) sipW 2666936..2667508 (-) 573 WP_003246088.1 signal peptidase I SipW -
  HIR77_RS14075 (HIR77_14095) tapA 2667492..2668253 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  HIR77_RS14080 (HIR77_14100) yqzG 2668525..2668851 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  HIR77_RS14085 (HIR77_14105) spoIITA 2668893..2669072 (-) 180 WP_003230176.1 YqzE family protein -
  HIR77_RS14090 (HIR77_14110) comGG 2669143..2669517 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  HIR77_RS14095 (HIR77_14115) comGF 2669518..2669901 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  HIR77_RS14100 (HIR77_14120) comGE 2669927..2670274 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  HIR77_RS14105 (HIR77_14125) comGD 2670258..2670689 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  HIR77_RS14110 (HIR77_14130) comGC 2670679..2670975 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  HIR77_RS14115 (HIR77_14135) comGB 2670989..2672026 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  HIR77_RS14120 (HIR77_14140) comGA 2672013..2673083 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  HIR77_RS14125 (HIR77_14145) corA 2673495..2674448 (-) 954 WP_004399136.1 magnesium transporter CorA -
  HIR77_RS14130 (HIR77_14150) yqhB 2674591..2675919 (+) 1329 WP_009967730.1 hemolysin family protein -
  HIR77_RS14135 (HIR77_14155) rsbRD 2675972..2676808 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=443108 HIR77_RS14115 WP_009967727.1 2670989..2672026(-) (comGB) [Bacillus subtilis subsp. subtilis str. 168]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=443108 HIR77_RS14115 WP_009967727.1 2670989..2672026(-) (comGB) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936