Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HLB22_RS00205 Genome accession   NZ_CP053101
Coordinates   47633..48982 (-) Length   449 a.a.
NCBI ID   WP_170267407.1    Uniprot ID   -
Organism   Staphylococcus aureus strain EDCC5398     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39218..80602 47633..48982 within 0


Gene organization within MGE regions


Location: 39218..80602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLB22_RS00165 (HLB22_00165) mecA 39218..41227 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HLB22_RS00170 (HLB22_00170) - 41324..42310 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HLB22_RS00175 (HLB22_00175) - 42282..42542 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  HLB22_RS00180 (HLB22_00180) - 42533..44140 (-) 1608 Protein_33 IS1182 family transposase -
  HLB22_RS00185 (HLB22_00185) - 44192..44698 (-) 507 WP_001092188.1 DUF1643 domain-containing protein -
  HLB22_RS00190 (HLB22_00190) - 44713..45024 (-) 312 WP_000700851.1 DUF960 family protein -
  HLB22_RS00195 (HLB22_00195) - 45111..45461 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HLB22_RS00200 (HLB22_00200) ccrB 45983..47611 (-) 1629 WP_001186608.1 recombinase family protein Machinery gene
  HLB22_RS00205 (HLB22_00205) ccrA 47633..48982 (-) 1350 WP_170267407.1 recombinase family protein Machinery gene
  HLB22_RS00210 (HLB22_00210) - 49216..51009 (-) 1794 WP_000195099.1 DUF927 domain-containing protein -
  HLB22_RS00215 (HLB22_00215) - 51009..51305 (-) 297 WP_000221966.1 single-stranded DNA-binding protein -
  HLB22_RS00220 (HLB22_00220) - 51477..52646 (+) 1170 Protein_41 hypothetical protein -
  HLB22_RS00225 (HLB22_00225) istB 52707..53474 (-) 768 WP_001095317.1 IS21-like element helper ATPase IstB -
  HLB22_RS00230 (HLB22_00230) istA 53486..54721 (-) 1236 WP_001215400.1 IS21 family transposase -
  HLB22_RS00235 (HLB22_00235) - 54889..55371 (-) 483 WP_000714379.1 hypothetical protein -
  HLB22_RS00240 (HLB22_00240) - 55446..58598 (-) 3153 WP_000080558.1 DEAD/DEAH box helicase -
  HLB22_RS00245 (HLB22_00245) - 58865..59467 (-) 603 WP_000908952.1 hypothetical protein -
  HLB22_RS00250 (HLB22_00250) - 60011..60430 (+) 420 WP_000039763.1 DUF1433 domain-containing protein -
  HLB22_RS00255 (HLB22_00255) - 60661..61404 (-) 744 WP_014667668.1 LysR family transcriptional regulator -
  HLB22_RS00260 (HLB22_00260) - 61495..62028 (+) 534 WP_000543162.1 DUF4865 family protein -
  HLB22_RS00265 (HLB22_00265) - 62382..63242 (-) 861 WP_001052508.1 LysR family transcriptional regulator -
  HLB22_RS00270 (HLB22_00270) - 63355..63906 (+) 552 WP_000627029.1 flavodoxin family protein -
  HLB22_RS00275 (HLB22_00275) - 63899..65119 (+) 1221 WP_000130758.1 MFS transporter -
  HLB22_RS00280 (HLB22_00280) - 65183..65479 (-) 297 WP_000201639.1 TfoX/Sxy family protein -
  HLB22_RS00285 (HLB22_00285) - 65528..65731 (-) 204 WP_000389764.1 DUF6007 family protein -
  HLB22_RS00290 (HLB22_00290) - 66040..67026 (+) 987 WP_000662036.1 tRNA-dihydrouridine synthase -
  HLB22_RS00295 (HLB22_00295) - 67084..67656 (-) 573 WP_000540837.1 TetR/AcrR family transcriptional regulator -
  HLB22_RS00300 (HLB22_00300) - 67804..69321 (+) 1518 WP_000107170.1 MDR family MFS transporter -
  HLB22_RS00305 (HLB22_00305) - 69323..69880 (+) 558 WP_000633422.1 DUF4811 domain-containing protein -
  HLB22_RS00310 (HLB22_00310) - 70143..70610 (-) 468 WP_000575287.1 hypothetical protein -
  HLB22_RS00315 (HLB22_00315) - 70603..71310 (-) 708 WP_000667078.1 hypothetical protein -
  HLB22_RS00320 (HLB22_00320) - 71423..74551 (-) 3129 WP_000577171.1 type I restriction endonuclease subunit R -
  HLB22_RS00325 (HLB22_00325) - 74535..75767 (-) 1233 WP_000394002.1 restriction endonuclease subunit S -
  HLB22_RS00330 (HLB22_00330) - 75757..77271 (-) 1515 WP_000190897.1 class I SAM-dependent DNA methyltransferase -
  HLB22_RS00335 (HLB22_00335) - 77310..77786 (-) 477 WP_001670222.1 Pycsar system effector family protein -
  HLB22_RS00340 (HLB22_00340) pycC 77792..79063 (-) 1272 WP_000368661.1 Pycsar phage resistance system cytidylate cyclase PycC -
  HLB22_RS00345 (HLB22_00345) - 79259..79756 (-) 498 WP_000051479.1 hypothetical protein -
  HLB22_RS00350 (HLB22_00350) - 80225..80602 (-) 378 WP_000687965.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52655.27 Da        Isoelectric Point: 10.0062

>NTDB_id=443017 HLB22_RS00205 WP_170267407.1 47633..48982(-) (ccrA) [Staphylococcus aureus strain EDCC5398]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
HGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQRIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=443017 HLB22_RS00205 WP_170267407.1 47633..48982(-) (ccrA) [Staphylococcus aureus strain EDCC5398]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
CACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACGCATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.327

100

0.973
  ccrA Staphylococcus aureus COL

75.501

100

0.755