Detailed information    

insolico Bioinformatically predicted

Overview

Name   vraR   Type   Regulator
Locus tag   HK402_RS10115 Genome accession   NZ_CP053075
Coordinates   2023482..2024111 (-) Length   209 a.a.
NCBI ID   WP_000153535.1    Uniprot ID   W8TS97
Organism   Staphylococcus aureus strain SA01     
Function   repress expression of competence genes (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1987421..2048548 2023482..2024111 within 0


Gene organization within MGE regions


Location: 1987421..2048548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HK402_RS09920 (HK402_09930) perR 1988009..1988455 (-) 447 WP_000110007.1 peroxide-responsive transcriptional repressor PerR -
  HK402_RS09925 (HK402_09935) - 1988552..1989502 (-) 951 WP_000869446.1 phosphoglycerate dehydrogenase -
  HK402_RS09930 (HK402_09940) bcp 1989508..1989963 (-) 456 WP_000939521.1 thioredoxin-dependent thiol peroxidase -
  HK402_RS09935 (HK402_09945) - 1990044..1991333 (+) 1290 WP_001011595.1 glutamate-1-semialdehyde 2,1-aminomutase -
  HK402_RS09940 (HK402_09950) - 1991626..1992720 (+) 1095 WP_001236371.1 aromatic acid exporter family protein -
  HK402_RS09945 (HK402_09955) - 1992810..1992901 (-) 92 Protein_1929 hypothetical protein -
  HK402_RS09950 (HK402_09960) - 1992910..1994646 (-) 1737 WP_000597238.1 SAV1866 family putative multidrug efflux ABC transporter -
  HK402_RS09955 (HK402_09965) - 1994937..1995479 (-) 543 WP_000251253.1 DUF402 domain-containing protein -
  HK402_RS09960 (HK402_09970) mutY 1995782..1996819 (-) 1038 WP_000284765.1 A/G-specific adenine glycosylase -
  HK402_RS09965 (HK402_09975) - 1996971..1997948 (+) 978 WP_000379098.1 metal-dependent hydrolase -
  HK402_RS09970 (HK402_09980) - 1998209..1999045 (-) 837 WP_000886462.1 membrane protein -
  HK402_RS09975 (HK402_09985) - 1999054..2000571 (-) 1518 WP_000535834.1 ABC transporter ATP-binding protein -
  HK402_RS09980 (HK402_09990) - 2000586..2000900 (-) 315 WP_000435806.1 YfhH family protein -
  HK402_RS09985 (HK402_09995) recX 2000878..2001696 (-) 819 WP_001124422.1 recombination regulator RecX -
  HK402_RS09990 (HK402_10000) sgtB 2001956..2002765 (-) 810 WP_000830380.1 monofunctional peptidoglycan glycosyltransferase SgtB -
  HK402_RS09995 (HK402_10005) - 2003105..2003620 (-) 516 WP_000163283.1 type 1 glutamine amidotransferase domain-containing protein -
  HK402_RS10000 (HK402_10010) - 2003751..2003912 (-) 162 WP_001005407.1 SE1561 family protein -
  HK402_RS10005 (HK402_10015) yfkAB 2004136..2005287 (+) 1152 WP_001089621.1 radical SAM/CxCxxxxC motif protein YfkAB -
  HK402_RS10010 (HK402_10020) - 2005410..2005694 (+) 285 WP_000134547.1 hypothetical protein -
  HK402_RS10015 (HK402_10025) - 2005845..2006165 (+) 321 WP_000805733.1 DUF961 family protein -
  HK402_RS10020 (HK402_10030) - 2006179..2006481 (+) 303 WP_000386891.1 hypothetical protein -
  HK402_RS10025 (HK402_10035) - 2006656..2007747 (+) 1092 WP_000172943.1 replication initiation factor domain-containing protein -
  HK402_RS10030 (HK402_10040) - 2007808..2008863 (+) 1056 WP_000692002.1 conjugal transfer protein -
  HK402_RS10035 (HK402_10045) - 2008868..2009128 (+) 261 WP_000015639.1 TcpD family membrane protein -
  HK402_RS10040 (HK402_10050) - 2009140..2009523 (+) 384 WP_000358144.1 TcpE family conjugal transfer membrane protein -
  HK402_RS10045 (HK402_10055) - 2009558..2012053 (+) 2496 WP_001049264.1 ATP-binding protein -
  HK402_RS10050 (HK402_10060) - 2012107..2013465 (+) 1359 WP_001251209.1 FtsK/SpoIIIE domain-containing protein -
  HK402_RS10055 (HK402_10065) - 2013470..2015316 (+) 1847 Protein_1951 hypothetical protein -
  HK402_RS10060 (HK402_10070) - 2015306..2016352 (+) 1047 WP_000247465.1 CHAP domain-containing protein -
  HK402_RS10065 (HK402_10075) - 2016359..2016949 (+) 591 WP_000810443.1 hypothetical protein -
  HK402_RS10070 (HK402_10080) - 2017005..2017385 (+) 381 WP_170172377.1 cystatin-like fold lipoprotein -
  HK402_RS10075 (HK402_10085) - 2017469..2017972 (+) 504 WP_000746373.1 transposase -
  HK402_RS10080 (HK402_10090) - 2018001..2018489 (+) 489 WP_142377520.1 IS30 family transposase -
  HK402_RS10085 (HK402_10095) - 2018902..2019432 (-) 531 WP_000184383.1 acyl-CoA thioesterase -
  HK402_RS10090 (HK402_10100) - 2019522..2020778 (-) 1257 WP_001795108.1 aminopeptidase -
  HK402_RS10095 (HK402_10105) - 2020781..2020981 (-) 201 WP_000180460.1 DUF1128 family protein -
  HK402_RS10100 (HK402_10110) - 2021128..2021592 (+) 465 WP_000228666.1 low molecular weight protein-tyrosine-phosphatase -
  HK402_RS10105 (HK402_10115) - 2021599..2021874 (+) 276 WP_170172378.1 YtxH domain-containing protein -
  HK402_RS10110 (HK402_10120) - 2022152..2023369 (+) 1218 WP_000037075.1 YihY/virulence factor BrkB family protein -
  HK402_RS10115 (HK402_10125) vraR 2023482..2024111 (-) 630 WP_000153535.1 two-component system response regulator VraR Regulator
  HK402_RS10120 (HK402_10130) vraS 2024101..2025144 (-) 1044 WP_001017131.1 sensor histidine kinase Regulator
  HK402_RS10125 (HK402_10135) liaF 2025141..2025842 (-) 702 WP_000149064.1 cell wall-active antibiotics response protein LiaF -
  HK402_RS10130 (HK402_10140) - 2025857..2026243 (-) 387 WP_001110179.1 hypothetical protein -
  HK402_RS10135 (HK402_10145) map 2026460..2027218 (-) 759 WP_000636142.1 type I methionyl aminopeptidase -
  HK402_RS10140 (HK402_10150) - 2027749..2028735 (+) 987 WP_000999717.1 aromatic acid exporter family protein -
  HK402_RS10145 (HK402_10155) - 2029166..2029579 (-) 414 WP_001557448.1 hypothetical protein -
  HK402_RS10150 (HK402_10160) - 2029608..2029718 (-) 111 WP_001790257.1 hypothetical protein -
  HK402_RS10155 (HK402_10165) - 2029804..2030535 (-) 732 WP_000544969.1 type 1 glutamine amidotransferase -
  HK402_RS10160 (HK402_10170) murT 2030537..2031850 (-) 1314 WP_001250343.1 lipid II isoglutaminyl synthase subunit MurT -
  HK402_RS10165 (HK402_10175) ftnA 2032142..2032642 (+) 501 WP_000949467.1 H-type ferritin FtnA -
  HK402_RS10170 (HK402_10180) - 2032724..2032816 (-) 93 WP_001790679.1 hypothetical protein -
  HK402_RS10175 (HK402_10185) - 2033031..2033585 (+) 555 WP_000613738.1 3'-5' exonuclease -
  HK402_RS10180 (HK402_10190) dinB 2033653..2034723 (-) 1071 WP_000140172.1 DNA polymerase IV -
  HK402_RS10185 (HK402_10195) - 2034970..2035500 (-) 531 WP_000548781.1 DUF3267 domain-containing protein -
  HK402_RS10190 (HK402_10200) rlmD 2035670..2037031 (-) 1362 WP_001147865.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  HK402_RS10195 (HK402_10205) - 2037112..2038059 (-) 948 WP_170172379.1 diacylglycerol kinase -
  HK402_RS10200 (HK402_10210) - 2038641..2038787 (-) 147 WP_001789566.1 hypothetical protein -
  HK402_RS10205 (HK402_10215) gatB 2038827..2040254 (-) 1428 WP_162675320.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  HK402_RS10210 (HK402_10220) gatA 2040267..2041724 (-) 1458 WP_000027917.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA -
  HK402_RS10215 (HK402_10225) gatC 2041726..2042028 (-) 303 WP_000170162.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  HK402_RS10220 (HK402_10230) putP 2042395..2043933 (+) 1539 WP_170172380.1 sodium/proline symporter PutP -
  HK402_RS10225 (HK402_10235) - 2044022..2045221 (-) 1200 WP_000831700.1 CamS family sex pheromone protein -
  HK402_RS10230 (HK402_10240) ligA 2045234..2047237 (-) 2004 WP_000774556.1 NAD-dependent DNA ligase LigA -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 23559.32 Da        Isoelectric Point: 5.3935

>NTDB_id=442844 HK402_RS10115 WP_000153535.1 2023482..2024111(-) (vraR) [Staphylococcus aureus strain SA01]
MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVL
MLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=442844 HK402_RS10115 WP_000153535.1 2023482..2024111(-) (vraR) [Staphylococcus aureus strain SA01]
ATGACGATTAAAGTATTGTTTGTGGATGATCATGAAATGGTACGTATAGGAATTTCAAGTTATCTATCAACGCAAAGTGA
TATTGAAGTAGTTGGTGAAGGCGCTTCTGGTAAAGAAGCAATTGCCAAAGCCCATGAGTTGAAGCCAGATTTAATTTTAA
TGGATTTACTTATGGAAGACATGGATGGTGTAGAAGCGACGACTCAGATTAAAAAAGATTTACCGCAAATTAAAGTATTA
ATGTTAACTAGTTTTATTGAAGATAAAGAGGTATATCGTGCATTAGATGCAGGTGTCGATAGTTACATTTTAAAAACAAC
AAGTGCAAAAGATATCGCCGATGCAGTTCGTAAAACTTCTAGAGGAGAATCTGTTTTTGAACCGGAAGTTTTAGTGAAAA
TGCGTAACCGTATGAAAAAGCGCGCAGAGTTATATGAAATGCTTACAGAACGAGAAATGGAAATATTATTATTGATTGCG
AAAGGTTACTCAAATCAAGAAATTGCTAGTGCATCGCATATTACTATTAAAACGGTTAAGACACATGTGAGTAACATTTT
AAGTAAGTTAGAAGTGCAAGATAGAACACAAGCTGTCATCTATGCATTCCAACATAATTTAATTCAATAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(5-116)

Bacterial regulatory proteins, luxR family (GerE)

(147-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8TS97

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  vraR Staphylococcus aureus N315

100

100

1
  degU Bacillus subtilis subsp. subtilis str. 168

34.821

100

0.373