Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DSM104440_RS00350 Genome accession   NZ_CP053073
Coordinates   79427..80890 (+) Length   487 a.a.
NCBI ID   WP_171159691.1    Uniprot ID   A0A6M4H1B8
Organism   Usitatibacter palustris strain Swamp67     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74427..85890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104440_RS00325 (DSM104440_00066) - 75916..76929 (+) 1014 WP_171159680.1 KpsF/GutQ family sugar-phosphate isomerase -
  DSM104440_RS00330 (DSM104440_00067) - 76935..77441 (+) 507 WP_171159682.1 HAD-IIIA family hydrolase -
  DSM104440_RS00335 (DSM104440_00068) lptC 77438..78013 (+) 576 WP_171159684.1 LPS export ABC transporter periplasmic protein LptC -
  DSM104440_RS00340 (DSM104440_00069) lptA 78013..78606 (+) 594 WP_171159686.1 lipopolysaccharide transport periplasmic protein LptA -
  DSM104440_RS00345 (DSM104440_00070) lptB 78611..79393 (+) 783 WP_171159689.1 LPS export ABC transporter ATP-binding protein -
  DSM104440_RS00350 (DSM104440_00071) rpoN/rpoN1 79427..80890 (+) 1464 WP_171159691.1 RNA polymerase factor sigma-54 Machinery gene
  DSM104440_RS00355 (DSM104440_00072) raiA 80916..81242 (+) 327 WP_171159693.1 ribosome-associated translation inhibitor RaiA -
  DSM104440_RS00360 (DSM104440_00073) - 81249..81734 (+) 486 WP_171159695.1 PTS sugar transporter subunit IIA -
  DSM104440_RS00365 (DSM104440_00074) hprK 81703..82668 (+) 966 WP_171159697.1 HPr(Ser) kinase/phosphatase -
  DSM104440_RS00370 (DSM104440_00075) rapZ 82668..83501 (+) 834 WP_171159699.1 RNase adapter RapZ -
  DSM104440_RS00375 (DSM104440_00076) - 83504..84121 (+) 618 WP_171159701.1 LON peptidase substrate-binding domain-containing protein -
  DSM104440_RS00380 (DSM104440_00077) mutY 84024..85124 (-) 1101 WP_171159703.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54439.60 Da        Isoelectric Point: 5.4032

>NTDB_id=442727 DSM104440_RS00350 WP_171159691.1 79427..80890(+) (rpoN/rpoN1) [Usitatibacter palustris strain Swamp67]
MKHSLQLKLSQHLTLTPQLQQSIRLLQLSTVELNQELERYLAENPLLEREDIPQEESLPAKPGTNPETEAERDGDAEGDG
ESQAASAPETHEDRGNDDVPSMGDDMGSGVRRDSGDRDDFTQFAAAEPTLQDHLLAQLALLSLTERDRNIAALLVGHLDE
DGYFPRDLAELRETMAEAIPELEPEEIDIARRHIQFLEPTGVGARDVPECLALQLRTMPVQTPHRDGAIALVTNHLDVLA
ARDFNRLKRLLGVDEDELRDIRALILTLDPKPGRAFGAGDVRYVVPDVVVRKSSGRWTATLNRDAMPRLRINKMYADILQ
ASRDNGAKHLAGQLQEARWLIKNVQQRFDTILRVTQAIVDRQKNYFEHGEVAMRPLVLREIAEAVGLHESTISRVTTQKY
MFTPRGILELKYFFGSHVSTDTGGSCSATAIRALIKQLVAAEDGKKPLSDHRISDILAQQGIQVARRTVAKYREALQIQP
ANLRKSI

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=442727 DSM104440_RS00350 WP_171159691.1 79427..80890(+) (rpoN/rpoN1) [Usitatibacter palustris strain Swamp67]
ATGAAGCACAGCCTCCAGCTCAAGCTCTCACAGCACCTCACCCTCACGCCGCAGTTGCAGCAATCGATCCGATTGCTCCA
GCTCTCGACGGTGGAGCTCAATCAGGAATTGGAGCGCTACCTCGCCGAGAACCCGCTGCTCGAGCGCGAGGACATCCCCC
AGGAAGAATCCCTGCCGGCCAAGCCCGGGACGAACCCCGAAACCGAGGCTGAACGCGACGGCGACGCCGAGGGCGACGGC
GAATCGCAGGCTGCCTCCGCCCCCGAGACCCACGAAGACCGCGGCAACGATGATGTCCCCTCGATGGGCGACGACATGGG
CAGCGGTGTCCGCCGCGACAGCGGCGACCGCGACGACTTCACGCAGTTTGCGGCGGCCGAGCCCACGCTCCAGGACCACC
TGCTCGCGCAGCTCGCTCTGCTCTCGCTGACGGAGCGCGACCGCAACATTGCCGCGCTCCTCGTGGGCCACCTCGACGAA
GACGGTTATTTCCCGCGCGACCTCGCGGAGTTGCGCGAGACGATGGCCGAGGCGATCCCCGAGCTCGAGCCCGAGGAAAT
CGACATCGCGCGGCGCCACATCCAGTTCCTCGAGCCCACCGGGGTCGGTGCGCGCGACGTCCCGGAATGCCTCGCGCTCC
AGTTGCGCACGATGCCCGTGCAAACACCGCATCGCGACGGCGCGATCGCGCTCGTCACCAACCACCTCGATGTCCTCGCC
GCGCGCGACTTCAACCGGTTGAAACGCCTGCTGGGCGTGGACGAAGACGAGCTGCGCGACATCCGCGCGCTCATCCTCAC
GCTCGATCCCAAGCCGGGCCGCGCCTTCGGTGCGGGCGATGTGCGCTACGTCGTACCCGATGTCGTGGTGCGGAAATCCA
GCGGTCGCTGGACCGCCACGCTCAACCGCGACGCGATGCCGCGCCTGCGGATCAACAAGATGTACGCCGACATCCTGCAG
GCCTCGCGCGACAACGGCGCCAAGCACCTCGCGGGGCAGCTCCAGGAAGCGCGCTGGCTCATCAAGAACGTGCAGCAGCG
CTTCGACACGATCCTCCGGGTCACGCAGGCCATCGTCGACAGGCAGAAGAATTATTTCGAGCACGGCGAGGTCGCCATGC
GCCCGCTCGTGCTGCGCGAGATCGCCGAGGCGGTGGGTCTCCACGAATCCACGATCTCGCGCGTCACCACGCAGAAGTAC
ATGTTCACGCCGCGCGGCATCCTCGAGCTGAAGTACTTCTTCGGCAGCCACGTTTCCACCGACACGGGAGGCTCGTGCTC
CGCCACGGCCATCCGCGCGCTCATCAAGCAGCTTGTCGCCGCAGAGGATGGCAAGAAGCCCCTCTCGGACCACCGAATCT
CCGACATCCTCGCGCAGCAGGGCATCCAGGTCGCACGGCGCACCGTTGCGAAGTACCGCGAGGCGTTGCAGATACAGCCG
GCAAATCTCCGCAAGTCGATCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4H1B8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.663

100

0.556