Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DSM104443_RS00360 Genome accession   NZ_CP053069
Coordinates   81851..83332 (+) Length   493 a.a.
NCBI ID   WP_171088740.1    Uniprot ID   A0A6M4GTS7
Organism   Usitatibacter rugosus strain 0125_3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76851..88332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104443_RS00335 (DSM104443_00067) - 78330..79334 (+) 1005 WP_171088735.1 KpsF/GutQ family sugar-phosphate isomerase -
  DSM104443_RS00340 (DSM104443_00068) - 79335..79856 (+) 522 WP_171088736.1 HAD-IIIA family hydrolase -
  DSM104443_RS00345 (DSM104443_00069) lptC 79862..80431 (+) 570 WP_171088737.1 LPS export ABC transporter periplasmic protein LptC -
  DSM104443_RS00350 (DSM104443_00070) lptA 80442..81029 (+) 588 WP_171088738.1 lipopolysaccharide transport periplasmic protein LptA -
  DSM104443_RS00355 (DSM104443_00071) lptB 81032..81814 (+) 783 WP_171088739.1 LPS export ABC transporter ATP-binding protein -
  DSM104443_RS00360 (DSM104443_00072) rpoN/rpoN1 81851..83332 (+) 1482 WP_171088740.1 RNA polymerase factor sigma-54 Machinery gene
  DSM104443_RS00365 (DSM104443_00073) raiA 83371..83700 (+) 330 WP_171088741.1 ribosome-associated translation inhibitor RaiA -
  DSM104443_RS00370 (DSM104443_00074) - 83721..84206 (+) 486 WP_171088742.1 PTS sugar transporter subunit IIA -
  DSM104443_RS00375 (DSM104443_00075) hprK 84175..85140 (+) 966 WP_171088743.1 HPr(Ser) kinase/phosphatase -
  DSM104443_RS00380 (DSM104443_00076) rapZ 85145..85978 (+) 834 WP_171088744.1 RNase adapter RapZ -
  DSM104443_RS00385 (DSM104443_00077) - 85982..86602 (+) 621 WP_212756851.1 LON peptidase substrate-binding domain-containing protein -
  DSM104443_RS00390 mutY 86502..87635 (-) 1134 WP_171088745.1 A/G-specific adenine glycosylase -
  DSM104443_RS00400 (DSM104443_00079) - 87840..88121 (+) 282 WP_171088746.1 2TM domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 55095.39 Da        Isoelectric Point: 5.4161

>NTDB_id=442657 DSM104443_RS00360 WP_171088740.1 81851..83332(+) (rpoN/rpoN1) [Usitatibacter rugosus strain 0125_3]
MKHSLQLKLSQHLTLTPQLQQSIRLLQLSTVELNQELERYLAENPLLERADLAPDEPTSVIGDRGAAPEGSPTSSAQEEP
AAEAHEREEREERSDRMDEMPMRDNGGGEEMGGSGSRRGDDDREDFTQFAAAEVTLHDHLTQQLSLTSLTERDKRLAALV
IGNIDEDGYLQQTLQELLDNAIDEFAELELEEIAIALKHVQNMDPAGVAARDVSECLTLQLKSLPTDTPYLAPAVELCAK
HLDVLAARDFNRLKRTLGVDEDELREIRALIVSLDPKPGRSFGSGDIRYVVPDVVVRRVGTRWVAALNRDAMPRLRVNKM
YADILQASRENGAKHLAGQLQEARWLIKNVQQRFDTILRVTQSIVDRQKNFFEHGEVAMRPLVLREIAEAVGLHESTISR
VTTQKYMLTARGIFELKYFFGSHVSTDTGGACSATAIRALIKQLVAAEDGKKPLSDHRISDILAQQGIQVARRTVAKYRE
AMHIQPANLRKSI

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=442657 DSM104443_RS00360 WP_171088740.1 81851..83332(+) (rpoN/rpoN1) [Usitatibacter rugosus strain 0125_3]
ATGAAGCACAGCCTCCAGCTAAAGCTCAGCCAGCACCTCACCCTCACGCCGCAGTTGCAGCAGTCGATCCGCTTGCTGCA
GCTTTCGACGGTGGAGCTGAACCAGGAGCTCGAGCGCTATCTCGCCGAGAACCCCCTGCTGGAGCGCGCCGACCTGGCGC
CCGACGAACCCACGAGCGTGATCGGCGATCGCGGCGCCGCCCCCGAGGGCTCCCCGACCTCCTCGGCCCAGGAGGAGCCC
GCCGCCGAGGCGCACGAGCGAGAGGAACGCGAAGAGCGCAGCGACCGCATGGACGAAATGCCCATGCGAGACAACGGCGG
CGGCGAGGAGATGGGCGGCAGCGGATCGCGCCGCGGCGACGACGACCGCGAGGACTTCACGCAATTCGCCGCGGCCGAGG
TCACGCTCCACGACCACCTCACCCAGCAGCTTTCGCTCACCTCGCTCACGGAGCGGGACAAGCGCCTGGCCGCCCTCGTC
ATCGGCAACATCGACGAAGACGGCTACCTGCAGCAGACCCTCCAGGAGCTCCTCGACAACGCCATCGACGAATTCGCCGA
GCTGGAGCTGGAGGAAATCGCGATCGCGCTGAAGCACGTCCAGAACATGGACCCCGCCGGCGTCGCGGCGCGCGACGTGA
GCGAATGCCTCACCCTGCAGCTGAAGTCGCTGCCCACCGACACGCCCTACCTCGCGCCCGCGGTCGAGCTCTGCGCCAAG
CACCTGGACGTGCTTGCCGCCCGCGACTTCAATCGCCTCAAGCGCACGCTGGGCGTGGACGAAGACGAGCTGCGCGAGAT
CCGCGCCCTCATCGTCTCGCTCGACCCCAAGCCCGGCCGCAGCTTCGGCAGCGGCGACATCCGCTACGTGGTGCCCGATG
TCGTCGTGCGCCGCGTCGGCACGCGCTGGGTCGCGGCGCTGAACCGCGACGCGATGCCGCGCCTGCGCGTGAACAAGATG
TACGCGGATATCCTCCAGGCATCCCGCGAGAACGGCGCCAAGCATCTGGCCGGCCAGCTCCAGGAAGCGCGCTGGCTCAT
CAAGAACGTGCAGCAGCGCTTCGACACGATCCTGCGAGTCACGCAGTCGATCGTGGACAGGCAGAAGAACTTCTTCGAGC
ACGGCGAGGTCGCGATGCGCCCGCTGGTGCTGCGCGAGATCGCGGAGGCCGTCGGCCTGCATGAATCCACCATCTCGCGC
GTGACCACGCAAAAGTACATGCTGACCGCGCGCGGCATCTTCGAGCTGAAGTACTTCTTCGGCAGCCACGTCTCCACGGA
TACCGGCGGCGCCTGCTCGGCCACGGCGATCCGCGCGCTGATCAAGCAGCTCGTCGCGGCGGAGGACGGCAAGAAGCCCC
TCTCCGACCACCGCATCTCGGACATCCTCGCGCAACAGGGCATCCAGGTCGCGCGGCGCACCGTAGCAAAGTACCGCGAG
GCGATGCACATCCAGCCGGCGAACCTGCGCAAGTCGATCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(126-321)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4GTS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.516

100

0.556