Detailed information NTG0442262

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: IRJ28_RS17455
• Genome accession: NZ_CP064096
• Coordinates: 3333773..3334894 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain N1142-3at
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3328773..3339894

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ28_RS17435	znuC	3329282..3329977 (-)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-
IRJ28_RS17440	znuA	3330033..3330992 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
IRJ28_RS17445	ycdG	3331180..3332865 (-)	1686	WP_003234736.1	alpha-glucosidase	-
IRJ28_RS17450	ycdF	3332890..3333666 (-)	777	WP_003246415.1	SDR family oxidoreductase	-
IRJ28_RS17455	rapF	3333773..3334894 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
IRJ28_RS17460	cwlK	3335017..3335520 (+)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
IRJ28_RS17465	ycdC	3335528..3336889 (-)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
IRJ28_RS17470	ycdB	3337076..3338494 (-)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
IRJ28_RS17475	-	3338668..3338799 (+)	132	WP_003246334.1	hypothetical protein	-
IRJ28_RS17480	ycdA	3338822..3339886 (+)	1065	WP_003234750.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=442262 IRJ28_RS17455 WP_003246478.1 3333773..3334894(-) (rapF) [Bacillus subtilis strain N1142-3at]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=442262 IRJ28_RS17455 WP_003246478.1 3333773..3334894(-) (rapF) [Bacillus subtilis strain N1142-3at]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405