Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IRJ28_RS05190 Genome accession   NZ_CP064096
Coordinates   986539..986949 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain N1142-3at     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 981539..991949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS05155 arsR 981702..982019 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  IRJ28_RS05160 yqcK 982080..982520 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  IRJ28_RS05165 acr3 982543..983583 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  IRJ28_RS05170 arsC 983595..984014 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  IRJ28_RS05175 - 984369..984547 (+) 179 Protein_1005 hypothetical protein -
  IRJ28_RS05180 spoIVCA 984505..985965 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  IRJ28_RS05185 - 985996..986343 (-) 348 Protein_1007 sigma-70 family RNA polymerase sigma factor -
  IRJ28_RS05190 nucA/comI 986539..986949 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  IRJ28_RS05195 yqeB 986982..987704 (-) 723 WP_010886572.1 hypothetical protein -
  IRJ28_RS05200 gnd 987956..988849 (+) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  IRJ28_RS05205 yqeD 988868..989494 (-) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  IRJ28_RS05210 cwlH 989681..990433 (+) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  IRJ28_RS05215 yqeF 990685..991416 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  IRJ28_RS05220 - 991722..991862 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=442165 IRJ28_RS05190 WP_009967785.1 986539..986949(+) (nucA/comI) [Bacillus subtilis strain N1142-3at]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=442165 IRJ28_RS05190 WP_009967785.1 986539..986949(+) (nucA/comI) [Bacillus subtilis strain N1142-3at]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529