NTDB

Overview

Name	radA	Type	Machinery gene
Locus tag	GV829_RS08140	Genome accession	NZ_CP053015
Coordinates	1693806..1695173 (+)	Length	455 a.a.
NCBI ID	WP_169948118.1	Uniprot ID	A0A6M4AX22
Organism	Sphingomonas lacunae strain CSW-10
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1688806..1700173

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GV829_RS08105 (GV829_08110)	rsmI	1688819..1689661 (-)	843	WP_169945658.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
GV829_RS08110 (GV829_08115)	-	1689660..1690850 (+)	1191	WP_246202780.1	penicillin-binding protein activator	-
GV829_RS08115 (GV829_08120)	-	1690935..1691207 (-)	273	WP_169945660.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GV829_RS08120 (GV829_08125)	-	1691191..1691418 (-)	228	WP_169945662.1	TraY domain-containing protein	-
GV829_RS08125 (GV829_08130)	fsa	1691471..1692124 (-)	654	WP_169945664.1	fructose-6-phosphate aldolase	-
GV829_RS08130 (GV829_08135)	-	1692290..1692916 (+)	627	WP_169945666.1	DUF2490 domain-containing protein	-
GV829_RS08135 (GV829_08140)	-	1692999..1693700 (-)	702	WP_169945668.1	DUF4197 domain-containing protein	-
GV829_RS08140 (GV829_08145)	radA	1693806..1695173 (+)	1368	WP_169948118.1	DNA repair protein RadA	Machinery gene
GV829_RS08145 (GV829_08150)	-	1695127..1695738 (+)	612	WP_246202781.1	CvpA family protein	-
GV829_RS08150 (GV829_08155)	-	1695743..1696183 (+)	441	WP_169945670.1	iron-sulfur cluster assembly scaffold protein	-
GV829_RS08155 (GV829_08160)	-	1696180..1697973 (+)	1794	WP_169945672.1	cation:proton antiporter	-
GV829_RS08160 (GV829_08165)	-	1697963..1698319 (+)	357	WP_169945674.1	DUF423 domain-containing protein	-
GV829_RS08165 (GV829_08170)	-	1698607..1698750 (-)	144	WP_169945676.1	DUF2256 domain-containing protein	-
GV829_RS08170 (GV829_08175)	aroB	1698753..1699865 (-)	1113	WP_169945678.1	3-dehydroquinate synthase	-

Sequence

Protein

Download Length: 455 a.a. Molecular weight: 47489.29 Da Isoelectric Point: 6.7204

                                >NTDB_id=442130 GV829_RS08140 WP_169948118.1 1693806..1695173(+) (radA) [Sphingomonas lacunae strain CSW-10]

MAKPKRTFVCQACGSSQNRWQGQCPDCGEWNTLAEEAPATVFSQKHDLSAGGRPIELESLSGADTMPARMHSGVGEFDRA

VGGGLVAGSATLIGGDPGIGKSTLLLQVAARLALAGKSVAYVSGEEAAGQVRLRAARLGYANAPVSLASATSVRDILTTM

GQGAAPDLLIIDSIQTMHSDLIEGAPGTVSQVRASAQELIRFAKDRGTAVILVGHVTKDGSLAGPRVLEHMVDTVLAFEG

ERSHQYRILRAVKNRFGGTDEIGVFAMAQEGLQEVTNPSALFLTDRHGEVAPGTAVFPALEGTRPVLVEIQALTLRLASG

ATPRRAVVGWDSGRLAMILAVLEARCGLSLSSAEVYLNVAGGYRLTDPAADMAVAAAIISAFAERPIPADAVIFGEIALS

GELRPVAHAPLRLKEAGKLGFARAIIPAHAAPDAGVRLQTHDRLADFVDHLLGRG

Nucleotide

Download Length: 1368 bp

                                >NTDB_id=442130 GV829_RS08140 WP_169948118.1 1693806..1695173(+) (radA) [Sphingomonas lacunae strain CSW-10]

ATGGCCAAGCCCAAACGCACCTTTGTCTGCCAGGCGTGCGGCAGCTCGCAAAACCGCTGGCAAGGGCAATGCCCCGACTG

CGGCGAATGGAACACGCTCGCCGAAGAAGCCCCTGCCACTGTCTTCTCGCAAAAGCATGACCTGTCGGCCGGTGGCCGCC

CGATTGAGCTGGAAAGCCTCTCGGGCGCGGACACCATGCCGGCGCGCATGCACAGCGGTGTCGGCGAATTTGACCGTGCG

GTTGGCGGTGGACTGGTTGCCGGCTCCGCGACGCTGATCGGCGGTGATCCCGGCATAGGCAAGTCGACGTTGTTGTTGCA

GGTCGCGGCCCGCCTCGCGCTGGCTGGCAAGTCGGTGGCCTATGTCTCGGGAGAGGAAGCCGCCGGGCAGGTTCGCCTGC

GCGCCGCCCGGCTGGGCTATGCCAATGCGCCGGTCAGCCTCGCCTCGGCCACGTCTGTGCGCGACATTTTGACCACGATG

GGGCAGGGCGCTGCCCCTGACCTCCTCATCATCGATTCGATCCAGACGATGCATTCGGACCTGATCGAGGGTGCGCCCGG

CACCGTCAGCCAGGTCCGCGCCTCGGCGCAGGAGTTGATACGCTTTGCCAAGGATCGCGGCACCGCCGTCATCCTCGTCG

GCCATGTCACCAAGGACGGCAGCCTCGCCGGCCCGCGCGTGCTCGAACATATGGTCGACACAGTTCTGGCGTTCGAGGGG

GAACGCAGCCACCAGTATCGCATCCTCCGCGCGGTCAAAAACCGCTTTGGCGGCACCGACGAGATCGGCGTGTTCGCCAT

GGCGCAGGAGGGGTTGCAGGAAGTCACCAATCCCTCCGCCCTGTTCCTCACCGACCGGCACGGGGAGGTCGCGCCGGGCA

CCGCTGTCTTCCCGGCGCTGGAGGGCACGCGCCCTGTCCTCGTCGAGATACAGGCGCTGACCCTGCGCCTCGCCAGCGGG

GCCACCCCGCGCCGTGCCGTCGTCGGCTGGGATTCGGGCCGCCTCGCGATGATCCTTGCGGTGCTGGAGGCGCGCTGCGG

CCTCTCGCTCTCTTCGGCGGAGGTCTATCTCAACGTTGCCGGTGGCTATCGCCTGACCGATCCGGCGGCGGATATGGCGG

TAGCGGCGGCGATCATTTCGGCCTTTGCCGAACGGCCCATCCCCGCCGATGCCGTCATTTTCGGGGAGATTGCGCTGTCG

GGCGAATTGCGCCCGGTCGCCCACGCACCGCTCCGGCTCAAGGAAGCGGGCAAGCTCGGTTTCGCCCGGGCGATCATTCC

CGCCCATGCCGCGCCCGATGCCGGCGTCCGCCTGCAAACGCATGATCGCCTCGCCGATTTCGTTGACCATCTGCTGGGCA

GGGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(346-428)

AAA domain (AAA_25)

(81-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6M4AX22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
radA	Bacillus subtilis subsp. subtilis str. 168	50.229	95.824	0.481
radA	Streptococcus mitis NCTC 12261	47.222	94.945	0.448
radA	Streptococcus pneumoniae Rx1	46.991	94.945	0.446
radA	Streptococcus pneumoniae D39	46.991	94.945	0.446
radA	Streptococcus pneumoniae R6	46.991	94.945	0.446
radA	Streptococcus pneumoniae TIGR4	46.991	94.945	0.446
radA	Streptococcus mitis SK321	46.991	94.945	0.446

Detailed information NTG0442130