Detailed information NTG0442050

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: IRJ27_RS08285
• Genome accession: NZ_CP064095
• Coordinates: 1653829..1654950 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain N1303-2Ay
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1648829..1659950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ27_RS08265	znuC	1649338..1650033 (-)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-
IRJ27_RS08270	znuA	1650089..1651048 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
IRJ27_RS08275	ycdG	1651236..1652921 (-)	1686	WP_003234736.1	alpha-glucosidase	-
IRJ27_RS08280	ycdF	1652946..1653722 (-)	777	WP_003246415.1	SDR family oxidoreductase	-
IRJ27_RS08285	rapF	1653829..1654950 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
IRJ27_RS08290	cwlK	1655073..1655576 (+)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
IRJ27_RS08295	ycdC	1655584..1656945 (-)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
IRJ27_RS08300	ycdB	1657132..1658550 (-)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
IRJ27_RS08305	-	1658724..1658855 (+)	132	WP_003246334.1	hypothetical protein	-
IRJ27_RS08310	ycdA	1658878..1659942 (+)	1065	WP_003234750.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=442050 IRJ27_RS08285 WP_003246478.1 1653829..1654950(-) (rapF) [Bacillus subtilis strain N1303-2Ay]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=442050 IRJ27_RS08285 WP_003246478.1 1653829..1654950(-) (rapF) [Bacillus subtilis strain N1303-2Ay]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405