Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HJC54_RS22140 Genome accession   NZ_CP052867
Coordinates   2057242..2058753 (+) Length   503 a.a.
NCBI ID   WP_015877038.1    Uniprot ID   A0AAQ0BTD2
Organism   Burkholderia glumae strain HN1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2052242..2063753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HJC54_RS22115 (HJC54_22130) kdsD 2053460..2054443 (+) 984 WP_169512160.1 arabinose 5-phosphate isomerase KdsD -
  HJC54_RS22120 (HJC54_22135) - 2054443..2054982 (+) 540 WP_015877034.1 HAD family hydrolase -
  HJC54_RS22125 (HJC54_22140) lptC 2054986..2055600 (+) 615 WP_015877035.1 LPS export ABC transporter periplasmic protein LptC -
  HJC54_RS22130 (HJC54_22145) lptA 2055619..2056290 (+) 672 WP_015877036.1 lipopolysaccharide transport periplasmic protein LptA -
  HJC54_RS22135 (HJC54_22150) lptB 2056287..2057063 (+) 777 WP_015877037.1 LPS export ABC transporter ATP-binding protein -
  HJC54_RS22140 (HJC54_22155) rpoN/rpoN1 2057242..2058753 (+) 1512 WP_015877038.1 RNA polymerase factor sigma-54 Machinery gene
  HJC54_RS22145 (HJC54_22160) raiA 2058905..2059264 (+) 360 WP_015877039.1 ribosome-associated translation inhibitor RaiA -
  HJC54_RS22150 (HJC54_22165) ptsN 2059481..2059993 (+) 513 WP_015877040.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HJC54_RS22155 (HJC54_22170) hprK 2060128..2061096 (+) 969 WP_015877041.1 HPr(Ser) kinase/phosphatase -
  HJC54_RS22160 (HJC54_22175) rapZ 2061171..2062064 (+) 894 WP_015877042.1 RNase adapter RapZ -
  HJC54_RS22165 (HJC54_22180) - 2062095..2062730 (+) 636 WP_017432629.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55057.11 Da        Isoelectric Point: 4.8365

>NTDB_id=441958 HJC54_RS22140 WP_015877038.1 2057242..2058753(+) (rpoN/rpoN1) [Burkholderia glumae strain HN1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSSAPEPMQ
GAPVGGDAPAGERSERDDGSGGDDYDSYSGSDGDASQWNLDDYGRTSGASDDDDLPPLQVHETSTSLRDHLSAQLRVTQA
SPRDRALITFLIESLDDDGYLGATLDEVRADLPEELEVELDELAAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTR
TLALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKSGQNWLAELNPEV
VPKLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=441958 HJC54_RS22140 WP_015877038.1 2057242..2058753(+) (rpoN/rpoN1) [Burkholderia glumae strain HN1]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCACCTCGCGCTGACTCCCCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAAAACGACGACGAGTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGACGCCGCCGTCCTCGGCGCCCGAGCCGATGCAG
GGTGCGCCCGTCGGCGGCGACGCGCCGGCCGGCGAGCGCAGCGAGCGCGACGACGGCTCGGGCGGCGACGACTACGACAG
CTACTCCGGCAGCGACGGCGATGCGAGCCAGTGGAACCTCGACGACTACGGCCGCACTTCGGGCGCCTCCGACGACGACG
ATCTCCCGCCGCTGCAGGTCCACGAGACCTCGACTTCGCTGCGCGACCACCTGTCGGCACAGCTGCGCGTCACCCAGGCA
AGCCCGCGCGACCGCGCGCTGATCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTGGGCGCGACGCTCGACGA
AGTCCGCGCCGACCTGCCCGAGGAGCTCGAGGTCGAGCTCGACGAGCTGGCCGCCGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCCGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGCCTGCAGCTGCTGCGACTCGATCCGTCCCCCACGCGC
ACGCTCGCGCTCGACATCGTTTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAA
GGCGAGCGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCCGCCTACGGCAAGG
CCGAGGCCGACTACGTGGTGCCCGACATCATGGTGAAGAAGAGCGGCCAGAACTGGCTCGCGGAGCTGAATCCGGAAGTC
GTGCCCAAGCTGCGCATCAACCACCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCCGGCGCCGGCTCGCTGAA
GCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGATTCGAGACCATCCTGCGCGTCGCGCAAGCAA
TCGTCGAGCGCCAGAAGAACTTTTTTGCACATGGCGAAATAGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAA
GTACTTCTTCGGGTCGCATGTCTCGACGGATACCGGCGGCGCCGCCTCATCCACGGCGATCCGCGCCCTCATCAAGCAAC
TGATAGGAGCCGAAGACCCGAAAACTCCTTTATCGGACAGCCGAATCGCCGAGCTGCTGGCGGAACAGGGATTCGTGGTC
GCGCGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.992

100

0.68