Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IRJ26_RS15950 Genome accession   NZ_CP064094
Coordinates   3033314..3033724 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain N1108-5at     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3028314..3038724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ26_RS15915 arsR 3028477..3028794 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  IRJ26_RS15920 yqcK 3028855..3029295 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  IRJ26_RS15925 acr3 3029318..3030358 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  IRJ26_RS15930 arsC 3030370..3030789 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  IRJ26_RS15935 - 3031144..3031322 (+) 179 Protein_3071 hypothetical protein -
  IRJ26_RS15940 spoIVCA 3031280..3032740 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  IRJ26_RS15945 - 3032771..3033118 (-) 348 Protein_3073 sigma-70 family RNA polymerase sigma factor -
  IRJ26_RS15950 nucA/comI 3033314..3033724 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  IRJ26_RS15955 yqeB 3033757..3034479 (-) 723 WP_010886572.1 hypothetical protein -
  IRJ26_RS15960 gnd 3034731..3035624 (+) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  IRJ26_RS15965 yqeD 3035643..3036269 (-) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  IRJ26_RS15970 cwlH 3036456..3037208 (+) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  IRJ26_RS15975 yqeF 3037460..3038191 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  IRJ26_RS15980 - 3038497..3038637 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=441933 IRJ26_RS15950 WP_009967785.1 3033314..3033724(+) (nucA/comI) [Bacillus subtilis strain N1108-5at]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=441933 IRJ26_RS15950 WP_009967785.1 3033314..3033724(+) (nucA/comI) [Bacillus subtilis strain N1108-5at]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529