Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HKK53_RS25300 Genome accession   NZ_CP052861
Coordinates   5498994..5500694 (-) Length   566 a.a.
NCBI ID   WP_169373030.1    Uniprot ID   -
Organism   Pseudomonas sp. ADAK7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5493994..5505694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKK53_RS25265 (HKK53_25265) - 5494344..5494970 (-) 627 WP_008154887.1 DUF1780 domain-containing protein -
  HKK53_RS25270 (HKK53_25270) - 5495105..5495794 (+) 690 WP_169373026.1 energy-coupling factor ABC transporter permease -
  HKK53_RS25275 (HKK53_25275) - 5495858..5496073 (+) 216 WP_008033212.1 hypothetical protein -
  HKK53_RS25280 (HKK53_25280) yacG 5496083..5496283 (-) 201 WP_149661048.1 DNA gyrase inhibitor YacG -
  HKK53_RS25285 (HKK53_25285) coaE 5496280..5496903 (-) 624 WP_169373027.1 dephospho-CoA kinase -
  HKK53_RS25290 (HKK53_25290) pilD 5496900..5497772 (-) 873 WP_169373028.1 A24 family peptidase Machinery gene
  HKK53_RS25295 (HKK53_25295) pilC 5497774..5498991 (-) 1218 WP_169373029.1 type II secretion system F family protein Machinery gene
  HKK53_RS25300 (HKK53_25300) pilB 5498994..5500694 (-) 1701 WP_169373030.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKK53_RS25305 (HKK53_25305) - 5500920..5501321 (+) 402 WP_169373031.1 pilin -
  HKK53_RS25310 (HKK53_25310) - 5501653..5502003 (+) 351 WP_169373032.1 BON domain-containing protein -
  HKK53_RS25315 (HKK53_25315) - 5502172..5502468 (-) 297 WP_169373033.1 DUF2845 domain-containing protein -
  HKK53_RS25320 (HKK53_25320) - 5502468..5505410 (-) 2943 WP_169373034.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62720.85 Da        Isoelectric Point: 6.1320

>NTDB_id=441896 HKK53_RS25300 WP_169373030.1 5498994..5500694(-) (pilB) [Pseudomonas sp. ADAK7]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNHISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDSHTTGLEDMADVDLDG
LDIESIDDKHQDSIPGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGVDALGYEPDQKALFMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKTPIEIPREALIKEGFPE
ERIGSFTIYEPVGCDHCNNGYKGRIGIYEVVKNTPDLQRLIMAEGNSLEIEIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=441896 HKK53_RS25300 WP_169373030.1 5498994..5500694(-) (pilB) [Pseudomonas sp. ADAK7]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGCAATCACATTTCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAAAGCCGGCAGGTGG
CGGAGATTGCCTCGGAACATTTCGGCATGGCCTTGCTGGATCTCCACTGCCTGGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGCGAGAAGCTGGTCCGCCAGCACCACGCCCTGCCCCTTTGGCGGCGCGGCAATAAATTGTTCGTGGGGATTTCCGA
CCCGACCAACCATCAGGCGATCAACGATATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATCCTGGTCGAAGACGACA
AGCTCAGCGACGCCATCGAGAAATTCTTCGACAGCCACACCACCGGCCTGGAAGACATGGCCGACGTTGACCTCGATGGC
CTGGACATTGAATCGATCGACGACAAGCATCAGGACTCCATCCCCGGCCAGGACGCCGATGATGCGCCGGTGGTGCGCTT
CGTCCACAAGATGCTGCTGGATGCGATCAAGAGCGGCTCTTCCGACTTGCACTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATTCTGCGCGAAGTGGCCAAGCCACCGATTCAACTGGCCGGCCGCATTGCCGCCCGGCTC
AAGGTCATGGCCAGCCTCGACATCTCCGAACGACGGCGACCGCAGGACGGACGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAGATGGGCGTCGACGCCCTCGGCTACGAGCCGGACCAGAAAGCCCTGTTCATGGCCGCGCTCAAGCAGCCCCAAGGG
ATGATCCTGGTCACCGGCCCCACCGGTTCGGGTAAAACCGTGTCGCTGTATACCGGGCTGAATATTCTTAATACCGTGGA
CATCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCAAGCAAG
GCCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGTGACCTC
GAAACCGCCGAAATAGCGATCAAGGCCGCCCAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTCACCCGCCTGCACAACATGGGCATCCCCGGCTTCAACATCGCTACGTCGGTGAGCCTGATCATCGCCCAGCGCC
TGGCACGTAAACTGTGCAGTCATTGCAAGACGCCGATCGAGATTCCCCGGGAGGCGCTGATCAAGGAGGGCTTCCCCGAG
GAACGCATCGGCTCTTTCACGATCTATGAGCCCGTCGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGAATCGGGAT
TTATGAAGTGGTGAAGAACACCCCGGACCTGCAACGGCTGATCATGGCCGAGGGCAACTCCCTGGAAATCGAGATCCAGA
TGCGCAAGGACGGCTTCAACGATCTGCGTACTTCGGGGCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.657

100

0.587
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.36

98.233

0.495
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491