Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HHK01_RS09960 Genome accession   NZ_CP052135
Coordinates   2019640..2020749 (-) Length   369 a.a.
NCBI ID   WP_322619366.1    Uniprot ID   -
Organism   Bacillus fonticola strain CS13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2014640..2025749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHK01_RS09915 - 2014723..2015535 (+) 813 WP_170007639.1 YqhG family protein -
  HHK01_RS09920 - 2015686..2015865 (-) 180 WP_170007640.1 YqzE family protein -
  HHK01_RS09925 - 2015896..2016417 (-) 522 WP_170007641.1 shikimate kinase -
  HHK01_RS09930 - 2016427..2016795 (-) 369 WP_170007642.1 hypothetical protein -
  HHK01_RS09935 - 2016789..2017250 (-) 462 WP_170007643.1 competence type IV pilus minor pilin ComGF -
  HHK01_RS09940 - 2017192..2017506 (-) 315 WP_170007644.1 hypothetical protein -
  HHK01_RS09945 - 2017487..2017939 (-) 453 WP_170007645.1 type II secretion system protein -
  HHK01_RS09950 comGC 2017920..2018237 (-) 318 WP_170007646.1 competence type IV pilus major pilin ComGC -
  HHK01_RS09955 - 2018625..2019662 (-) 1038 WP_170007647.1 type II secretion system F family protein -
  HHK01_RS09960 comGA 2019640..2020749 (-) 1110 WP_322619366.1 competence type IV pilus ATPase ComGA Machinery gene
  HHK01_RS09970 - 2021205..2021444 (+) 240 WP_170007648.1 DUF2626 domain-containing protein -
  HHK01_RS09975 - 2021500..2022141 (-) 642 WP_170007649.1 MBL fold metallo-hydrolase -
  HHK01_RS09980 - 2022309..2022479 (+) 171 WP_170007650.1 DUF2759 domain-containing protein -
  HHK01_RS09985 - 2022774..2023073 (-) 300 WP_170007651.1 group II intron maturase-specific domain-containing protein -
  HHK01_RS09990 mobV 2023393..2023956 (-) 564 WP_170007652.1 MobV family relaxase -
  HHK01_RS09995 - 2024188..2024562 (+) 375 WP_170007653.1 hypothetical protein -
  HHK01_RS15945 - 2024935..2025150 (-) 216 WP_407646904.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 42296.81 Da        Isoelectric Point: 9.1723

>NTDB_id=441447 HHK01_RS09960 WP_322619366.1 2019640..2020749(-) (comGA) [Bacillus fonticola strain CS13]
MCRKSKGTTRKVVKLIESIEQIASQLLEQAFQLQASDIHFSPRKHDTHIYFRLHDQLLLKHRWPHSDSERLISRFKFFAS
MDIGEKRRPQSGAHFLYVHDTPVHLRFSTLPSSFGESLVIRLMPQTTQVSLHELSLFPSITRKLCSLVQHAHGLLIFTGP
TGSGKTTSVYSLIQYASQIERRHVITLEDPVEQKSEDMLQVQVNEKAGITYATGLKAILRHDPDVILVGEIRDEETARVA
IRAAMTGHLVLTTMHTKDAAGAISRLQELGIPKQDIQETLIGVSAQRLVRRVCPICGENCRPYCPYHEKRRACICELLAG
RDLQHFYEGKQTVRPPYTLDRWLRKAICYGFVPEEEYKRWKLREEAPTP

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=441447 HHK01_RS09960 WP_322619366.1 2019640..2020749(-) (comGA) [Bacillus fonticola strain CS13]
ATGTGTCGAAAAAGTAAAGGGACGACAAGAAAGGTGGTGAAACTGATCGAATCAATCGAACAAATAGCATCCCAACTTTT
AGAGCAAGCGTTTCAATTGCAAGCTTCTGATATTCACTTTTCTCCTAGAAAGCACGATACACACATTTATTTCCGCCTTC
ATGATCAGCTTCTTTTGAAACACAGGTGGCCACATTCCGATAGTGAACGACTCATCAGTCGATTCAAATTTTTTGCCTCC
ATGGATATTGGGGAAAAACGAAGGCCGCAGAGTGGCGCTCACTTTCTTTACGTTCATGATACTCCTGTCCATTTACGCTT
TTCTACGCTACCCTCGTCTTTTGGCGAAAGTCTTGTCATCCGGTTGATGCCTCAAACTACCCAAGTATCCTTACATGAAT
TGTCATTGTTCCCATCCATCACGCGTAAACTTTGTTCTCTAGTCCAACACGCACACGGACTTCTCATATTCACAGGCCCA
ACTGGCAGTGGTAAGACCACTTCTGTGTACAGCCTCATTCAGTACGCCTCACAGATAGAGAGACGACACGTTATTACATT
GGAAGATCCCGTTGAACAAAAAAGTGAAGACATGCTTCAAGTCCAAGTTAATGAAAAGGCAGGTATTACTTATGCCACGG
GGTTGAAAGCAATTTTACGTCACGATCCTGACGTTATTCTTGTCGGTGAAATACGTGATGAAGAGACAGCGCGTGTGGCC
ATTCGGGCTGCAATGACTGGACATCTTGTGTTGACGACGATGCACACGAAAGATGCGGCTGGTGCTATTAGTCGGCTTCA
AGAGCTTGGGATACCGAAACAAGACATTCAAGAGACACTTATCGGCGTGAGTGCACAAAGGTTAGTGAGACGTGTTTGCC
CCATTTGCGGAGAAAATTGTCGTCCGTATTGTCCGTATCATGAAAAGAGAAGAGCGTGTATTTGCGAATTGTTAGCAGGT
CGCGACTTGCAACACTTCTATGAAGGAAAACAAACTGTCAGACCCCCATACACGTTGGATCGGTGGTTGCGCAAGGCAAT
TTGTTACGGGTTTGTCCCAGAAGAGGAATACAAAAGGTGGAAATTACGTGAAGAAGCTCCCACTCCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(26-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

51.831

96.206

0.499