Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GAS18_RS12065 Genome accession   NZ_CP052132
Coordinates   2650636..2652147 (-) Length   503 a.a.
NCBI ID   WP_015877038.1    Uniprot ID   A0AAQ0BTD2
Organism   Burkholderia glumae strain HN2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2645636..2657147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAS18_RS12040 (GAS18_12060) - 2646658..2647294 (-) 637 Protein_2368 LON peptidase substrate-binding domain-containing protein -
  GAS18_RS12045 (GAS18_12065) rapZ 2647325..2648218 (-) 894 WP_015877042.1 RNase adapter RapZ -
  GAS18_RS12050 (GAS18_12070) hprK 2648293..2649261 (-) 969 WP_015877041.1 HPr(Ser) kinase/phosphatase -
  GAS18_RS12055 (GAS18_12075) ptsN 2649396..2649908 (-) 513 WP_015877040.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GAS18_RS12060 (GAS18_12080) raiA 2650125..2650484 (-) 360 WP_015877039.1 ribosome-associated translation inhibitor RaiA -
  GAS18_RS12065 (GAS18_12085) rpoN/rpoN1 2650636..2652147 (-) 1512 WP_015877038.1 RNA polymerase factor sigma-54 Machinery gene
  GAS18_RS12070 (GAS18_12090) lptB 2652326..2653102 (-) 777 WP_015877037.1 LPS export ABC transporter ATP-binding protein -
  GAS18_RS12075 (GAS18_12095) lptA 2653099..2653770 (-) 672 WP_015877036.1 lipopolysaccharide transport periplasmic protein LptA -
  GAS18_RS12080 (GAS18_12100) lptC 2653789..2654403 (-) 615 WP_015877035.1 LPS export ABC transporter periplasmic protein LptC -
  GAS18_RS12085 (GAS18_12105) - 2654407..2654946 (-) 540 WP_015877034.1 HAD family hydrolase -
  GAS18_RS12090 (GAS18_12110) kdsD 2654946..2655929 (-) 984 WP_015877033.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55057.11 Da        Isoelectric Point: 4.8365

>NTDB_id=441420 GAS18_RS12065 WP_015877038.1 2650636..2652147(-) (rpoN/rpoN1) [Burkholderia glumae strain HN2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSSAPEPMQ
GAPVGGDAPAGERSERDDGSGGDDYDSYSGSDGDASQWNLDDYGRTSGASDDDDLPPLQVHETSTSLRDHLSAQLRVTQA
SPRDRALITFLIESLDDDGYLGATLDEVRADLPEELEVELDELAAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTR
TLALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKSGQNWLAELNPEV
VPKLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=441420 GAS18_RS12065 WP_015877038.1 2650636..2652147(-) (rpoN/rpoN1) [Burkholderia glumae strain HN2]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCACCTCGCGCTGACTCCCCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAAAACGACGACGAGTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGACGCCGCCGTCCTCGGCGCCCGAGCCGATGCAG
GGTGCGCCCGTCGGCGGCGACGCGCCGGCCGGCGAGCGCAGCGAGCGCGACGACGGCTCGGGCGGCGACGACTACGACAG
CTACTCCGGCAGCGACGGCGATGCGAGCCAGTGGAACCTCGACGACTACGGCCGCACTTCGGGCGCCTCCGACGACGACG
ATCTCCCGCCGCTGCAGGTCCACGAGACCTCGACTTCGCTGCGCGACCACCTGTCGGCACAGCTGCGCGTCACCCAGGCA
AGCCCGCGCGACCGCGCGCTGATCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTGGGCGCGACGCTCGACGA
AGTCCGCGCCGACCTGCCCGAGGAGCTCGAGGTCGAGCTCGACGAGCTGGCCGCCGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCCGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGCCTGCAGCTGCTGCGGCTCGATCCGTCCCCCACGCGC
ACGCTCGCGCTCGACATCGTTTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAA
GGCGAGCGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCCGCCTACGGCAAGG
CCGAGGCCGACTACGTGGTGCCCGACATCATGGTAAAGAAGAGCGGCCAGAACTGGCTCGCGGAGCTGAATCCGGAAGTC
GTGCCCAAGCTGCGCATCAACCACCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCCGGCGCCGGCTCGCTGAA
GCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGATTCGAGACCATCCTGCGCGTCGCGCAAGCAA
TCGTCGAGCGCCAGAAGAACTTTTTTGCACATGGCGAAATAGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAA
GTACTTCTTCGGGTCGCATGTCTCGACGGATACCGGCGGCGCCGCCTCATCCACGGCGATCCGCGCCCTCATCAAGCAAC
TGATAGGAGCCGAAGACCCGAAAACTCCTTTATCGGACAGCCGAATCGCCGAGCTGCTGGCGGAACAGGGATTCGTGGTC
GCGCGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.992

100

0.68