Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HED66_RS14020 Genome accession   NZ_CP051883
Coordinates   3086553..3087992 (-) Length   479 a.a.
NCBI ID   WP_059112774.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain SRW-OG1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3081553..3092992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HED66_RS13990 dagF 3081826..3082566 (+) 741 WP_169046918.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  HED66_RS13995 - 3082670..3084574 (+) 1905 WP_169046919.1 BglG family transcription antiterminator -
  HED66_RS14000 npr 3084593..3084865 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  HED66_RS14005 rapZ 3084886..3085752 (-) 867 WP_169046920.1 RNase adapter RapZ -
  HED66_RS14010 ptsN 3085795..3086241 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HED66_RS14015 hpf 3086244..3086531 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  HED66_RS14020 rpoN/rpoN1 3086553..3087992 (-) 1440 WP_059112774.1 RNA polymerase factor sigma-54 Machinery gene
  HED66_RS14025 lptB 3088058..3088783 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  HED66_RS14030 lptA 3088787..3089314 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  HED66_RS14035 lptC 3089301..3089861 (-) 561 WP_021140208.1 LPS export ABC transporter periplasmic protein LptC -
  HED66_RS14040 kdsC 3089858..3090412 (-) 555 WP_169046921.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HED66_RS14045 - 3090412..3091407 (-) 996 WP_034524103.1 KpsF/GutQ family sugar-phosphate isomerase -
  HED66_RS14050 mlaF 3091615..3092415 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53863.79 Da        Isoelectric Point: 4.3459

>NTDB_id=441330 HED66_RS14020 WP_059112774.1 3086553..3087992(-) (rpoN/rpoN1) [Aeromonas salmonicida strain SRW-OG1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=441330 HED66_RS14020 WP_059112774.1 3086553..3087992(-) (rpoN/rpoN1) [Aeromonas salmonicida strain SRW-OG1]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGATAGTCAGCTCGATTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACTTGGGAAGATGTCTATTCAGCTGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCTGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAACTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACTAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.776

100

0.486