Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HH195_RS09895 Genome accession   NZ_CP051754
Coordinates   2119152..2120513 (-) Length   453 a.a.
NCBI ID   WP_196370977.1    Uniprot ID   -
Organism   Sarcina sp. JB2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2114152..2125513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH195_RS09875 (HH195_09875) ispD 2115624..2116304 (-) 681 WP_055260200.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HH195_RS09880 (HH195_09880) - 2116310..2117407 (-) 1098 WP_196370976.1 PIN/TRAM domain-containing protein -
  HH195_RS09885 (HH195_09885) - 2117635..2118030 (+) 396 WP_055260198.1 hypothetical protein -
  HH195_RS09890 (HH195_09890) disA 2118069..2119136 (-) 1068 WP_055260197.1 DNA integrity scanning diadenylate cyclase DisA -
  HH195_RS09895 (HH195_09895) radA 2119152..2120513 (-) 1362 WP_196370977.1 DNA repair protein RadA Machinery gene
  HH195_RS09900 (HH195_09900) - 2120861..2121034 (+) 174 WP_195926319.1 hypothetical protein -
  HH195_RS09905 (HH195_09905) - 2121048..2121281 (+) 234 WP_055260195.1 glutaredoxin family protein -
  HH195_RS09910 (HH195_09910) - 2121320..2123776 (-) 2457 WP_055260194.1 ATP-dependent Clp protease ATP-binding subunit -
  HH195_RS09915 (HH195_09915) - 2123945..2124589 (+) 645 WP_196370978.1 MBL fold metallo-hydrolase -
  HH195_RS09920 (HH195_09920) - 2124638..2124808 (-) 171 WP_055260192.1 DUF362 domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50307.48 Da        Isoelectric Point: 6.5289

>NTDB_id=440867 HH195_RS09895 WP_196370977.1 2119152..2120513(-) (radA) [Sarcina sp. JB2]
MAKQKSIFICQNCGYESLKWLGKCPDCNSWNTMVEEIKDNNLKFTNINIEKKLDAVKKIGEIKSGEKERYNTKITELNRV
LGGGLVKGSMILISGDPGIGKSTLLLQTANNISKEYGKVLYVSGEESEEQIKIRGDRLGIDADELYIVSETNLDVINIYI
DQIKPIFVIIDSIQTVYKDSVSSAPGSVSQVKECSNSIMRIVKGNNIPLFIVAHVTKQGELAGPRVLEHMVDAVLSFEGE
RTEEFRILRTMKNRFGTTSEIGVFEMRQEGLIEIYDPSKMFLEDTSFNQEGSIVIGIIEGTRPILVEIQALVSETKAVMP
RRTAVGIENSRLSLILAVLEKKLKIPFYNTDIYVNVVGGLEIEGTTADLGIAIALISSVRGRVSKLERLLIIGEVGLTGE
IRPIANCERIINEAEKMGFLNAIIPYRNLEKVKNSKLNIIGVKTLKEAISKIF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=440867 HH195_RS09895 WP_196370977.1 2119152..2120513(-) (radA) [Sarcina sp. JB2]
GTGGCTAAACAAAAGAGTATATTTATATGCCAAAACTGCGGATATGAATCTCTTAAATGGCTTGGAAAATGTCCAGATTG
TAATAGCTGGAATACAATGGTTGAAGAAATAAAAGATAATAATTTAAAATTTACTAATATTAATATAGAAAAAAAACTTG
ATGCAGTAAAAAAGATAGGTGAAATAAAATCAGGAGAGAAGGAGAGATACAATACAAAGATTACTGAGCTTAACAGGGTA
TTGGGTGGAGGTCTTGTTAAGGGATCTATGATACTAATATCAGGAGATCCTGGAATTGGAAAGTCAACATTGCTTTTACA
AACTGCAAATAATATATCTAAAGAATATGGGAAGGTATTGTATGTTTCTGGAGAAGAATCAGAGGAACAAATAAAGATAA
GGGGAGATAGATTAGGGATAGATGCAGATGAATTATATATAGTATCTGAAACAAATTTAGATGTTATTAATATATATATA
GATCAAATTAAGCCTATTTTTGTTATAATAGATTCAATACAGACTGTATATAAAGATAGTGTTAGTTCTGCACCTGGAAG
TGTTTCTCAGGTAAAAGAATGTTCGAATTCTATAATGAGAATAGTTAAGGGAAATAATATACCTCTTTTTATAGTTGCTC
ATGTAACAAAGCAGGGAGAATTAGCTGGTCCTAGGGTTTTGGAGCATATGGTAGATGCAGTATTATCATTTGAGGGAGAG
AGAACAGAAGAATTTAGAATATTAAGAACTATGAAAAACAGGTTTGGAACGACATCAGAAATAGGGGTATTTGAAATGAG
ACAAGAAGGTCTTATAGAAATATATGATCCATCAAAAATGTTTTTAGAGGATACTTCGTTTAATCAAGAGGGCTCAATAG
TAATTGGAATTATAGAAGGAACTAGACCTATTCTTGTAGAAATACAGGCCTTGGTGAGCGAAACCAAAGCCGTAATGCCA
AGAAGAACAGCAGTTGGTATAGAGAATTCAAGATTAAGTTTAATTTTAGCTGTTCTAGAGAAAAAATTAAAAATTCCATT
TTATAATACTGATATATATGTAAATGTAGTTGGTGGACTTGAGATAGAAGGTACTACCGCAGATTTAGGTATTGCAATAG
CATTAATATCATCTGTTAGAGGTAGAGTGTCTAAATTGGAAAGATTATTAATTATTGGAGAAGTTGGATTAACAGGAGAA
ATTAGACCAATTGCCAATTGTGAAAGGATAATAAATGAAGCTGAGAAAATGGGATTTTTAAATGCCATAATACCTTATAG
AAATTTAGAAAAAGTAAAGAATTCTAAATTAAATATAATTGGAGTTAAAACTTTAAAGGAAGCTATAAGTAAGATATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.656

100

0.517
  radA Streptococcus mitis NCTC 12261

47.797

100

0.479
  radA Streptococcus pneumoniae Rx1

47.797

100

0.479
  radA Streptococcus pneumoniae D39

47.797

100

0.479
  radA Streptococcus pneumoniae R6

47.797

100

0.479
  radA Streptococcus pneumoniae TIGR4

47.797

100

0.479
  radA Streptococcus mitis SK321

47.577

100

0.477