Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IQS17_RS12355 Genome accession   NZ_CP063515
Coordinates   2499294..2499971 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain EH01-18-04-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494294..2504971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS17_RS12320 (IQS17_12340) gspG 2494774..2495211 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  IQS17_RS12325 (IQS17_12345) gspH 2495219..2495728 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  IQS17_RS12330 (IQS17_12350) gspI 2495725..2496102 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  IQS17_RS12335 (IQS17_12355) gspJ 2496095..2496682 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  IQS17_RS12340 (IQS17_12360) gspK 2496675..2497658 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  IQS17_RS12345 (IQS17_12365) gspL 2497673..2498836 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  IQS17_RS12350 (IQS17_12370) gspM 2498833..2499294 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  IQS17_RS12355 (IQS17_12375) pilD 2499294..2499971 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  IQS17_RS12360 (IQS17_12380) bfr 2500000..2500476 (-) 477 WP_000675504.1 bacterioferritin -
  IQS17_RS12365 (IQS17_12385) bfd 2500548..2500742 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IQS17_RS12370 (IQS17_12390) chiA 2500911..2503613 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=440478 IQS17_RS12355 WP_000178170.1 2499294..2499971(+) (pilD) [Escherichia coli strain EH01-18-04-A]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=440478 IQS17_RS12355 WP_000178170.1 2499294..2499971(+) (pilD) [Escherichia coli strain EH01-18-04-A]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382