Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HH212_RS05890 Genome accession   NZ_CP051685
Coordinates   1376174..1377673 (-) Length   499 a.a.
NCBI ID   WP_169434575.1    Uniprot ID   -
Organism   Massilia forsythiae strain GN2-R2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1371174..1382673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH212_RS05860 (HH212_05860) - 1371400..1371882 (+) 483 WP_169434569.1 PTS sugar transporter subunit IIA -
  HH212_RS05865 (HH212_05865) hprK 1371893..1372828 (+) 936 WP_169434570.1 HPr(Ser) kinase/phosphatase -
  HH212_RS05870 (HH212_05870) rapZ 1372877..1373740 (+) 864 WP_169434571.1 RNase adapter RapZ -
  HH212_RS05875 (HH212_05875) - 1373759..1374550 (-) 792 WP_169434572.1 APH(3') family aminoglycoside O-phosphotransferase -
  HH212_RS05880 (HH212_05880) - 1374579..1375661 (-) 1083 WP_169434573.1 enoyl-CoA hydratase/isomerase family protein -
  HH212_RS05885 (HH212_05885) hpf 1375732..1376097 (-) 366 WP_169434574.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HH212_RS05890 (HH212_05890) rpoN/rpoN1 1376174..1377673 (-) 1500 WP_169434575.1 RNA polymerase factor sigma-54 Machinery gene
  HH212_RS05895 (HH212_05895) lptB 1377676..1378458 (-) 783 WP_229217593.1 LPS export ABC transporter ATP-binding protein -
  HH212_RS05900 (HH212_05900) lptA 1378455..1379054 (-) 600 WP_169434576.1 lipopolysaccharide transport periplasmic protein LptA -
  HH212_RS05905 (HH212_05905) lptC 1379082..1379687 (-) 606 WP_169434577.1 LPS export ABC transporter periplasmic protein LptC -
  HH212_RS05910 (HH212_05910) - 1379692..1380237 (-) 546 WP_169434578.1 HAD family hydrolase -
  HH212_RS05915 (HH212_05915) - 1380237..1381235 (-) 999 WP_211172460.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54748.92 Da        Isoelectric Point: 4.8604

>NTDB_id=440457 HH212_RS05890 WP_169434575.1 1376174..1377673(-) (rpoN/rpoN1) [Massilia forsythiae strain GN2-R2]
MKQSLQLKTSQHLALTPQLQQSIRLLQLSTLELHQEIEQVLSDNPLLERLDDPLDRSVRLLADGAINNNAGTSTTPSNEA
PNAEANGQEGGAAAENGEGGEASGDEGGGAAEGADADWGETGRASGNGDDEDGRPQLEASPVSLREHLMEQLRLTCFAAR
DCALVELVIDALDDNGYLEEPLDDIHARLPQELEVEFDELRTALALVQSMDPPGVGARSAAECLALQIRRMHGVPLVTRR
MALVIVERYLSWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFAADVSDYVVPDVIVRRAKDGWSVQLNPEVM
PRLRVNSVYASLLKQGKSESQLSAQLQEAKWLIKNMRQRFDTILRVAQAIVDRQRNFFSHGAVAMRPLVLREIADTLGLH
ESTISRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRT
VAKYREALKIPPVSLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=440457 HH212_RS05890 WP_169434575.1 1376174..1377673(-) (rpoN/rpoN1) [Massilia forsythiae strain GN2-R2]
ATGAAACAGTCGCTGCAACTCAAGACCTCGCAACACCTGGCACTGACCCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGCACCAGGAAATCGAACAGGTGCTGAGCGATAACCCGCTGCTGGAACGCCTGGACGATCCGC
TCGACCGCTCGGTGCGCCTGCTGGCCGACGGCGCCATCAACAACAACGCCGGCACGTCGACGACCCCGTCGAACGAGGCG
CCCAACGCGGAAGCCAACGGCCAGGAAGGCGGCGCCGCTGCCGAGAACGGCGAAGGCGGCGAAGCCAGTGGCGACGAGGG
CGGCGGCGCAGCCGAAGGCGCGGATGCCGACTGGGGAGAGACGGGACGCGCCAGCGGCAATGGCGACGACGAGGACGGCC
GCCCGCAGCTGGAAGCCAGTCCGGTCTCGCTGCGCGAGCATTTGATGGAGCAGTTGCGCCTGACCTGCTTCGCCGCGCGC
GATTGCGCGCTGGTGGAACTGGTGATCGACGCGCTCGACGACAACGGTTACCTGGAAGAGCCGTTGGACGACATCCACGC
GCGCCTGCCGCAAGAGCTGGAAGTCGAGTTCGACGAGCTGCGCACCGCGCTGGCGCTGGTGCAGAGCATGGACCCGCCCG
GCGTCGGGGCGCGCAGCGCGGCCGAGTGCCTGGCGCTGCAGATCCGGCGCATGCACGGCGTGCCGCTGGTGACGCGGCGC
ATGGCCCTGGTCATCGTCGAGCGCTACCTGAGCTGGTTCGCGCAGCGCGAATTCAACAAGCTGAAAAAGGCGCTCGACTG
CGACGACGAAGACCTGCGCGAGGCGCAGGCGGTGATCCGCCAGTGCAATCCGCATCCGGGTGCGGCATTCGCAGCGGACG
TGTCCGACTACGTGGTGCCGGACGTGATCGTGCGCCGCGCCAAGGACGGCTGGAGCGTGCAATTGAATCCGGAAGTGATG
CCGCGCCTGCGCGTGAACAGCGTGTACGCGTCGCTGCTGAAACAGGGCAAGAGCGAGAGCCAATTGAGCGCGCAATTGCA
GGAAGCGAAGTGGCTGATCAAGAACATGCGCCAGCGTTTCGACACGATCCTGCGCGTGGCGCAAGCCATCGTCGATCGCC
AGCGCAACTTTTTCTCCCATGGCGCGGTTGCCATGCGTCCCCTTGTTTTACGCGAGATAGCTGATACACTAGGCCTACAC
GAGAGCACAATTTCTCGGGTTACAACTCAAAAATACATGCTGACGCCGCATGGCATGTTCGAGTTGAAGTACTTTTTTGG
GAGCCACGTTGCGACCGAGGCGGGCGGTGAAGCGTCCTCGACGGCAATCCGGGCTCTGATCGTGCAACTTACAGGAGCGG
AAGACCCCAAGAATCCCTTATCGGATAGCAAGATTGCGGACATGCTGGGGGAGCAGGGCATGGTGATTGCGCGCCGCACG
GTCGCCAAGTACCGTGAAGCCTTGAAGATTCCACCCGTCAGCCTCCGCAAATCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.289

100

0.601