Detailed information NTG0440451

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: HH212_RS03665
• Genome accession: NZ_CP051685
• Coordinates: 859481..860737 (+)
• Length: 418 a.a.
• NCBI ID: WP_169434137.1
• Uniprot ID: A0A7Z2VU53
• Organism: Massilia forsythiae strain GN2-R2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 854481..865737

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HH212_RS03650 (HH212_03650)	-	855769..856884 (-)	1116	WP_169434134.1	S41 family peptidase	-
HH212_RS03655 (HH212_03655)	-	857085..858785 (+)	1701	WP_169434135.1	thiamine pyrophosphate-binding protein	-
HH212_RS03660 (HH212_03660)	-	858860..859177 (-)	318	WP_169434136.1	hypothetical protein	-
HH212_RS03665 (HH212_03665)	pilA	859481..860737 (+)	1257	WP_169434137.1	signal recognition particle-docking protein FtsY	Machinery gene
HH212_RS03670 (HH212_03670)	-	860743..861432 (+)	690	WP_169434138.1	cell division ATP-binding protein FtsE	-
HH212_RS03675 (HH212_03675)	ftsX	861429..862349 (+)	921	WP_169434139.1	permease-like cell division protein FtsX	-
HH212_RS03680 (HH212_03680)	rpoH	862936..863826 (+)	891	WP_370663907.1	RNA polymerase sigma factor RpoH	-
HH212_RS03685 (HH212_03685)	-	864152..865036 (-)	885	WP_169434141.1	dienelactone hydrolase family protein	-
HH212_RS03690 (HH212_03690)	-	865222..865677 (+)	456	WP_169434142.1	MarR family winged helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 44510.10 Da
• Isoelectric Point: 4.9482

>NTDB_id=440451 HH212_RS03665 WP_169434137.1 859481..860737(+) (pilA) [Massilia forsythiae strain GN2-R2]
MFSFFKKKKPEADTPALAEAQSAALAAPAAPDEPSGQADAAPAAGPANFLRRLFGGGEAEPVAPVIPEAPAEPESPPARD
ATNIDPFAARIAADAAVLVNTYEGQLFPETAERPGSEQEKKKSWMARLKAGLSKTSSNLSLLFVGARIDEDLYEELESAL
LMSDAGMDATQFLLDSLRRKVKEEKLLDAAAVKGALRQLLVEMMRPLEKPLELGRHEPLVMMIAGVNGAGKTTTIGKLAK
HMQRFEQSVLLAAGDTFRAAAREQLMVWGQRNNVTVISQASGDPAAVAYDAVQSGKARGVDVVMVDTAGRLPTQLHLMEE
LKKIKRVIGKGMDDAPHEVLLVIDGNTGQNALAQVKAFDDALQLTGLVITKLDGTAKGGILAAIARTRPVPVYFIGVGEK
LEDLQPFSAEEFAEALLG

Nucleotide

• Length: 1257 bp

>NTDB_id=440451 HH212_RS03665 WP_169434137.1 859481..860737(+) (pilA) [Massilia forsythiae strain GN2-R2]
ATGTTTAGTTTCTTCAAAAAGAAAAAGCCCGAAGCAGACACGCCGGCGCTGGCGGAAGCGCAGTCCGCTGCGCTTGCCGC
ACCGGCCGCGCCGGACGAGCCCTCCGGGCAAGCGGACGCCGCGCCCGCCGCCGGCCCCGCGAATTTCCTGCGCCGCCTGT
TCGGCGGCGGCGAGGCCGAACCGGTCGCGCCCGTCATTCCCGAGGCGCCGGCCGAACCCGAATCGCCGCCGGCGCGCGAC
GCCACCAACATCGATCCGTTCGCCGCCAGGATCGCCGCCGATGCCGCCGTGCTGGTCAACACCTACGAAGGGCAACTCTT
TCCGGAAACGGCCGAACGTCCGGGCAGCGAGCAGGAAAAGAAGAAATCCTGGATGGCGCGCCTGAAGGCCGGCCTGTCCA
AGACCTCCAGCAACCTGTCGCTGCTGTTCGTCGGCGCGCGCATCGACGAAGACCTGTACGAAGAGCTGGAATCGGCACTG
CTGATGTCGGACGCCGGCATGGACGCCACCCAGTTCCTGCTCGACAGCCTGCGGCGCAAGGTCAAGGAAGAAAAATTGCT
GGACGCCGCCGCCGTCAAGGGCGCGCTCAGGCAATTACTGGTCGAGATGATGCGGCCGCTCGAAAAGCCGCTCGAACTGG
GCCGCCACGAACCGCTGGTGATGATGATCGCCGGCGTCAACGGCGCCGGCAAGACCACCACCATCGGCAAGCTGGCCAAG
CACATGCAGCGCTTCGAGCAATCGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGCGAGCAATTGATGGT
CTGGGGCCAGCGCAACAACGTCACCGTGATCTCGCAAGCCTCGGGCGACCCGGCCGCGGTCGCCTACGATGCGGTGCAGT
CGGGCAAGGCGCGCGGTGTGGACGTGGTCATGGTCGATACCGCCGGCCGCCTGCCGACCCAGCTGCACCTGATGGAAGAA
CTGAAAAAGATCAAGCGCGTGATCGGCAAGGGCATGGATGACGCCCCGCACGAAGTGCTGCTGGTAATCGACGGGAATAC
CGGCCAGAACGCGCTGGCGCAGGTGAAAGCCTTCGACGATGCGCTCCAATTGACCGGCCTGGTGATCACCAAGCTCGACG
GCACGGCGAAAGGCGGCATCCTGGCGGCGATCGCGCGCACCCGTCCGGTGCCGGTGTACTTCATCGGCGTCGGCGAAAAG
CTGGAGGACCTGCAACCCTTCAGCGCCGAAGAATTCGCCGAGGCGCTGCTGGGATAG

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (219-418)

• SRP54-type protein, helical bundle domain (SRP54_N) (145-198)

3D structure

• AlphaFold DB: A0A7Z2VU53

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	50.233	100	0.517