Detailed information NTG0044041

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: M013TW_RS07985
• Genome accession: NC_016928
• Coordinates: 1630064..1630561 (-)
• Length: 165 a.a.
• NCBI ID: WP_001838632.1
• Uniprot ID: A0A6A9GZK7
• Organism: Staphylococcus aureus subsp. aureus M013
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1625064..1635561

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M013TW_RS07960 (M013TW_1550)	gcvPB	1625226..1626698 (-)	1473	WP_000202177.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
M013TW_RS07965 (M013TW_1551)	gcvPA	1626691..1628037 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
M013TW_RS07970 (M013TW_1552)	gcvT	1628057..1629148 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
M013TW_RS07975 (M013TW_1553)	-	1629307..1629831 (-)	525	WP_001015116.1	shikimate kinase	-
M013TW_RS07980 (M013TW_03430)	-	1629821..1629967 (-)	147	WP_001789879.1	hypothetical protein	-
M013TW_RS07985 (M013TW_1554)	comGF	1630064..1630561 (-)	498	WP_001838632.1	competence type IV pilus minor pilin ComGF	Machinery gene
M013TW_RS07990 (M013TW_1555)	comGE	1630479..1630778 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
M013TW_RS07995 (M013TW_1556)	comGD	1630765..1631211 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
M013TW_RS08000 (M013TW_1557)	comGC	1631189..1631500 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
M013TW_RS08005 (M013TW_1558)	comGB	1631514..1632584 (-)	1071	WP_000776407.1	competence type IV pilus assembly protein ComGB	Machinery gene
M013TW_RS08010 (M013TW_1559)	comGA	1632556..1633530 (-)	975	WP_000697218.1	competence type IV pilus ATPase ComGA	Machinery gene
M013TW_RS08015 (M013TW_1560)	-	1633582..1634205 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
M013TW_RS08020 (M013TW_1561)	-	1634202..1634531 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
M013TW_RS08025 (M013TW_1562)	-	1634531..1635517 (-)	987	WP_000161308.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19184.87 Da
• Isoelectric Point: 10.2192

>NTDB_id=44041 M013TW_RS07985 WP_001838632.1 1630064..1630561(-) (comGF) [Staphylococcus aureus subsp. aureus M013]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=44041 M013TW_RS07985 WP_001838632.1 1630064..1630561(-) (comGF) [Staphylococcus aureus subsp. aureus M013]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A6A9GZK7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	96.364	100	0.964
comGF	Staphylococcus aureus MW2	96.364	100	0.964