Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IQS19_RS21735 Genome accession   NZ_CP063503
Coordinates   4494265..4494942 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EH09-18-41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4489265..4499942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS19_RS21705 (IQS19_21710) - 4489872..4490318 (+) 447 Protein_4289 type II secretion system protein GspD -
  IQS19_RS21710 (IQS19_21715) - 4490392..4491561 (+) 1170 Protein_4290 IS3-like element ISEc52 family transposase -
  IQS19_RS21715 (IQS19_21720) - 4491570..4491653 (+) 84 Protein_4291 type II secretion system protein J -
  IQS19_RS21720 (IQS19_21725) gspK 4491646..4492629 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IQS19_RS21725 (IQS19_21730) gspL 4492644..4493807 (+) 1164 WP_089075207.1 type II secretion system protein GspL -
  IQS19_RS21730 (IQS19_21735) gspM 4493804..4494265 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  IQS19_RS21735 (IQS19_21740) pilD 4494265..4494942 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IQS19_RS21740 (IQS19_21745) bfr 4494971..4495447 (-) 477 WP_000675504.1 bacterioferritin -
  IQS19_RS21745 (IQS19_21750) bfd 4495519..4495713 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IQS19_RS21750 (IQS19_21755) chiA 4495882..4498575 (-) 2694 WP_089075206.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=440347 IQS19_RS21735 WP_000178161.1 4494265..4494942(+) (pilD) [Escherichia coli strain EH09-18-41]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=440347 IQS19_RS21735 WP_000178161.1 4494265..4494942(+) (pilD) [Escherichia coli strain EH09-18-41]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378