Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   IQS20_RS16955 Genome accession   NZ_CP063499
Coordinates   3529579..3530247 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain EH10-18-47     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3524579..3535247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS20_RS16925 (IQS20_16930) yicC 3524598..3525461 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  IQS20_RS16930 (IQS20_16935) rph 3525588..3526304 (+) 717 WP_001247097.1 ribonuclease PH -
  IQS20_RS16935 (IQS20_16940) pyrE 3526370..3527011 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  IQS20_RS16940 (IQS20_16945) slmA 3527048..3527644 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  IQS20_RS16945 (IQS20_16950) dut 3527751..3528209 (-) 459 WP_000976070.1 dUTP diphosphatase -
  IQS20_RS16950 (IQS20_16955) coaBC 3528187..3529407 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  IQS20_RS16955 (IQS20_16960) radC 3529579..3530247 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  IQS20_RS16960 (IQS20_16965) rpmB 3530464..3530700 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  IQS20_RS16965 (IQS20_16970) rpmG 3530721..3530888 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  IQS20_RS16970 (IQS20_16975) mutM 3530986..3531795 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  IQS20_RS16975 (IQS20_16980) coaD 3531834..3532313 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  IQS20_RS16980 (IQS20_16985) waaA 3532321..3533598 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  IQS20_RS16985 (IQS20_16990) rfaQ 3534011..3535069 (+) 1059 WP_000360398.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=440246 IQS20_RS16955 WP_001298959.1 3529579..3530247(+) (radC) [Escherichia coli strain EH10-18-47]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=440246 IQS20_RS16955 WP_001298959.1 3529579..3530247(+) (radC) [Escherichia coli strain EH10-18-47]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496