Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HG715_RS00425 Genome accession   NZ_CP051649
Coordinates   76503..77879 (-) Length   458 a.a.
NCBI ID   WP_156661367.1    Uniprot ID   -
Organism   Lactobacillus acetotolerans strain LJ49     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 71503..82879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG715_RS00400 (HG715_00400) - 71522..72097 (-) 576 WP_170073360.1 sigma-70 family RNA polymerase sigma factor -
  HG715_RS00405 (HG715_00405) rlmB 72224..72979 (-) 756 WP_170073361.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  HG715_RS00410 (HG715_00410) - 72966..73409 (-) 444 WP_056969474.1 Mini-ribonuclease 3 -
  HG715_RS00415 (HG715_00415) cysS 73396..74826 (-) 1431 WP_170073362.1 cysteine--tRNA ligase -
  HG715_RS00420 (HG715_00420) gltX 74926..76425 (-) 1500 WP_060459234.1 glutamate--tRNA ligase -
  HG715_RS00425 (HG715_00425) radA 76503..77879 (-) 1377 WP_156661367.1 DNA repair protein RadA Machinery gene
  HG715_RS00430 (HG715_00430) - 77880..78437 (-) 558 WP_056969483.1 dUTP diphosphatase -
  HG715_RS00435 (HG715_00435) - 78559..78861 (+) 303 WP_170073363.1 hypothetical protein -
  HG715_RS00440 (HG715_00440) pepC 78942..80291 (+) 1350 WP_056969485.1 aminopeptidase C -
  HG715_RS00445 (HG715_00445) - 80344..81279 (-) 936 WP_170073364.1 AAA family ATPase -
  HG715_RS00450 (HG715_00450) - 81384..82061 (-) 678 WP_170073365.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  HG715_RS00455 (HG715_00455) - 82073..82255 (-) 183 WP_054681182.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50252.04 Da        Isoelectric Point: 8.7549

>NTDB_id=440223 HG715_RS00425 WP_156661367.1 76503..77879(-) (radA) [Lactobacillus acetotolerans strain LJ49]
MARIKTKYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSAKASPSRLIKKTGINEPVKLDKVKAEKDDRIATKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSKKYKVLYVSGEESASQIKLRANRLGLGASDMLLYPETDMHDI
REQINDVQPDFVVIDSIQTMNEPSLDTMTGSASQVREVTSELMKIAKMDAITIFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDKHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDERLPRSTGSSVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMSVASSYTNKEISATDCFVGEVGL
TGEVRRVNKIQARVNEAAKVGFKRIFIPRHNMYKGLKDLGIEVVPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=440223 HG715_RS00425 WP_156661367.1 76503..77879(-) (radA) [Lactobacillus acetotolerans strain LJ49]
ATGGCCCGTATAAAAACCAAATATAAGTGCCGTTCTTGTGGTTATATTTCTGCAAGTTATTTAGGGCGCTGTCCTAATTG
TGGCGCCTGGAATCAATTTGAAAAGGAAACGGAAGAAGTTCAAAAGCGTTCTGCCAAGGCCAGCCCCAGTCGGTTGATTA
AGAAAACAGGTATAAATGAGCCTGTTAAGTTAGATAAAGTTAAAGCGGAAAAAGACGACCGGATAGCCACTAAATCTGAA
GAATTGAACCGTGTTTTAGGTGGTGGTATTGTACCCGGCTCACTTGTATTAATCGGTGGGGATCCTGGAATAGGTAAATC
TACTTTGATGTTACAAATCATGAGTGATCTATCTAAAAAATATAAAGTTCTTTATGTTTCTGGAGAGGAATCAGCTAGTC
AGATTAAGCTGCGTGCCAATCGTTTAGGCTTAGGTGCTAGTGATATGCTTTTATATCCAGAAACAGATATGCACGATATT
CGTGAGCAAATTAATGATGTACAGCCTGATTTCGTTGTAATTGATTCTATCCAAACAATGAATGAGCCAAGTTTAGATAC
AATGACTGGCTCAGCTTCACAGGTTCGGGAAGTAACCAGTGAGTTGATGAAAATTGCTAAAATGGACGCAATTACTATTT
TTGTTATTGGCCATGTTACTAAAGAGGGAGCAATTGCTGGGCCAAAAATCATGGAGCATATGGTGGATACCGTTCTTTAC
TTTGAAGGAGATAAACACCACTCCTATCGAATCTTACATTCTGTAAAAAATCGTTTCGGTGCTGCTAATGAAATTGGGAT
GTTTGAGATGGAAAATGAGGGCTTAAAAGAAGTTACTAATCCTTCATCAATCTTTCTTGACGAACGTTTACCGCGTTCGA
CGGGTTCATCCGTTGTGGTTTCACTTGAAGGAACACGGCCTCTGTTAGCTGAAATTCAGGCGCTGGTCACACCAACAGCG
TTTGGTTATGCTAAGAGGACTACATCGGGTATTGATTATAATCGAGCCGCCCTTCTTTTGGCGGTACTTGAAAAAAGAGG
CAACCTTATGCTGCAAAATCAGGATGTATATCTTACTGCGACCGGTGGTATTAAATTAAACGAACCCGCGATTGATCTAG
CTATAACGATGTCTGTGGCATCGAGTTATACTAACAAAGAAATAAGTGCTACAGATTGTTTTGTAGGCGAAGTTGGTTTG
ACTGGAGAAGTTCGGCGGGTAAATAAAATTCAAGCTCGGGTTAATGAAGCAGCTAAGGTTGGGTTTAAACGGATTTTTAT
TCCCCGTCACAATATGTATAAGGGATTAAAAGATTTGGGAATTGAAGTCGTCCCAGTTTCGAGTATCCCGCAAGCTTTAA
AGCTTGTTTTTGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

58.206

99.782

0.581
  radA Streptococcus mitis NCTC 12261

58.206

99.782

0.581
  radA Streptococcus pneumoniae Rx1

58.206

99.782

0.581
  radA Streptococcus pneumoniae D39

58.206

99.782

0.581
  radA Streptococcus pneumoniae R6

58.206

99.782

0.581
  radA Streptococcus pneumoniae TIGR4

58.206

99.782

0.581
  radA Bacillus subtilis subsp. subtilis str. 168

54.902

100

0.55