Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IQS15_RS10820 Genome accession   NZ_CP063487
Coordinates   2279262..2279939 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EF7-18-51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2274262..2284939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS15_RS10805 (IQS15_10810) chiA 2275629..2278322 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  IQS15_RS10810 (IQS15_10815) bfd 2278491..2278685 (+) 195 WP_072712057.1 bacterioferritin-associated ferredoxin -
  IQS15_RS10815 (IQS15_10820) bfr 2278757..2279233 (+) 477 WP_000675504.1 bacterioferritin -
  IQS15_RS10820 (IQS15_10825) pilD 2279262..2279939 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IQS15_RS10825 (IQS15_10830) gspM 2279939..2280400 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IQS15_RS10830 (IQS15_10835) gspL 2280397..2281560 (-) 1164 WP_072712059.1 type II secretion system protein GspL -
  IQS15_RS10835 (IQS15_10840) gspK 2281575..2282558 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IQS15_RS10840 (IQS15_10845) gspJ 2282551..2283138 (-) 588 WP_072712062.1 type II secretion system minor pseudopilin GspJ -
  IQS15_RS10845 (IQS15_10850) gspI 2283131..2283508 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IQS15_RS10850 (IQS15_10855) gspH 2283505..2284014 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  IQS15_RS10855 (IQS15_10860) gspG 2284022..2284459 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=440067 IQS15_RS10820 WP_000178161.1 2279262..2279939(-) (pilD) [Escherichia coli strain EF7-18-51]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=440067 IQS15_RS10820 WP_000178161.1 2279262..2279939(-) (pilD) [Escherichia coli strain EF7-18-51]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378