Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IQS24_RS19900 Genome accession   NZ_CP063473
Coordinates   4096460..4097137 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EM18-18-42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4091460..4102137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS24_RS19865 (IQS24_19870) gspG 4091940..4092377 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  IQS24_RS19870 (IQS24_19875) gspH 4092385..4092894 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  IQS24_RS19875 (IQS24_19880) gspI 4092891..4093268 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IQS24_RS19880 (IQS24_19885) gspJ 4093261..4093848 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IQS24_RS19885 (IQS24_19890) gspK 4093841..4094824 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  IQS24_RS19890 (IQS24_19895) gspL 4094839..4096002 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  IQS24_RS19895 (IQS24_19900) gspM 4095999..4096460 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  IQS24_RS19900 (IQS24_19905) pilD 4096460..4097137 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IQS24_RS19905 (IQS24_19910) bfr 4097166..4097642 (-) 477 WP_000675504.1 bacterioferritin -
  IQS24_RS19910 (IQS24_19915) bfd 4097714..4097908 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IQS24_RS19915 (IQS24_19920) chiA 4098077..4100769 (-) 2693 Protein_3924 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=439762 IQS24_RS19900 WP_000178161.1 4096460..4097137(+) (pilD) [Escherichia coli strain EM18-18-42]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=439762 IQS24_RS19900 WP_000178161.1 4096460..4097137(+) (pilD) [Escherichia coli strain EM18-18-42]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378