Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HF085_RS02365 Genome accession   NZ_CP051479
Coordinates   445513..446487 (+) Length   324 a.a.
NCBI ID   WP_000697217.1    Uniprot ID   A0AAN2D5C1
Organism   Staphylococcus aureus strain NGA76     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440513..451487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF085_RS02330 (HF085_07770) rpmG 440973..441122 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  HF085_RS02335 (HF085_07765) - 441331..441870 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  HF085_RS02340 (HF085_07760) - 441882..443345 (+) 1464 WP_001017865.1 rhomboid family intramembrane serine protease -
  HF085_RS02345 (HF085_07755) - 443326..443529 (+) 204 WP_000087561.1 YqgQ family protein -
  HF085_RS02350 (HF085_07750) - 443526..444512 (+) 987 WP_000161314.1 ROK family glucokinase -
  HF085_RS02355 (HF085_07745) - 444512..444841 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  HF085_RS02360 (HF085_07740) - 444838..445461 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  HF085_RS02365 (HF085_07735) comGA 445513..446487 (+) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  HF085_RS02370 (HF085_07730) comGB 446459..447529 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  HF085_RS02375 (HF085_07725) comGC 447543..447854 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  HF085_RS02380 (HF085_07720) comGD 447832..448278 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  HF085_RS02385 (HF085_07715) comGE 448265..448564 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  HF085_RS02390 (HF085_07710) comGF 448482..448979 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  HF085_RS02395 (HF085_07705) - 449076..449222 (+) 147 WP_001792109.1 hypothetical protein -
  HF085_RS02400 (HF085_07700) - 449212..449736 (+) 525 WP_001015120.1 shikimate kinase -
  HF085_RS02405 (HF085_07695) gcvT 449895..450986 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36869.90 Da        Isoelectric Point: 8.8516

>NTDB_id=438775 HF085_RS02365 WP_000697217.1 445513..446487(+) (comGA) [Staphylococcus aureus strain NGA76]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIAPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=438775 HF085_RS02365 WP_000697217.1 445513..446487(+) (comGA) [Staphylococcus aureus strain NGA76]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGCACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTGGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAATTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997