Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HC659_RS01975 Genome accession   NZ_CP051465
Coordinates   371625..372074 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis subsp. subtilis strain UCMB5121     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366625..377074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC659_RS01940 (HC659_03840) yckA 366900..367580 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  HC659_RS01945 (HC659_03850) yckB 367590..368453 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  HC659_RS01950 - 368615..368807 (+) 193 Protein_342 hypothetical protein -
  HC659_RS01955 (HC659_03860) - 368843..369163 (+) 321 Protein_343 RDD family protein -
  HC659_RS01960 (HC659_03870) yckD 369245..369577 (+) 333 WP_015252849.1 YckD family protein -
  HC659_RS01965 (HC659_03880) bglC 369730..371163 (+) 1434 WP_032722901.1 6-phospho-beta-glucosidase -
  HC659_RS01970 (HC659_03890) nin/comJ 371200..371598 (-) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  HC659_RS01975 (HC659_03900) nucA/comI 371625..372074 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  HC659_RS01980 (HC659_03910) tlpC 372242..373963 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  HC659_RS01985 (HC659_03920) hxlB 374074..374631 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  HC659_RS01990 (HC659_03930) hxlA 374637..375269 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  HC659_RS01995 (HC659_03940) hxlR 375502..375864 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=438533 HC659_RS01975 WP_003234608.1 371625..372074(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5121]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=438533 HC659_RS01975 WP_003234608.1 371625..372074(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5121]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98