Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HC661_RS01955 Genome accession   NZ_CP051463
Coordinates   364216..364653 (-) Length   145 a.a.
NCBI ID   WP_015416783.1    Uniprot ID   -
Organism   Bacillus velezensis strain UCMB5140     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359216..369653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC661_RS01930 (HC661_03780) - 360235..361371 (+) 1137 WP_015416779.1 zinc-dependent alcohol dehydrogenase -
  HC661_RS01935 (HC661_03790) - 361386..361820 (+) 435 WP_012116767.1 RDD family protein -
  HC661_RS01940 (HC661_03800) - 361893..362216 (+) 324 WP_007409357.1 YckD family protein -
  HC661_RS01945 (HC661_03810) - 362320..363756 (+) 1437 WP_015416781.1 family 1 glycosylhydrolase -
  HC661_RS01950 (HC661_03820) nin/comJ 363797..364195 (-) 399 WP_015416782.1 competence protein ComJ Regulator
  HC661_RS01955 (HC661_03830) nucA/comI 364216..364653 (-) 438 WP_015416783.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HC661_RS01960 (HC661_03840) hxlB 365011..365568 (-) 558 WP_015416784.1 6-phospho-3-hexuloisomerase -
  HC661_RS01965 (HC661_03850) hxlA 365565..366200 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  HC661_RS01970 (HC661_03860) - 366432..366794 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16038.34 Da        Isoelectric Point: 8.4850

>NTDB_id=438381 HC661_RS01955 WP_015416783.1 364216..364653(-) (nucA/comI) [Bacillus velezensis strain UCMB5140]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLLKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=438381 HC661_RS01955 WP_015416783.1 364216..364653(-) (nucA/comI) [Bacillus velezensis strain UCMB5140]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTGAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634