Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   IM720_RS30515 Genome accession   NZ_CP063233
Coordinates   6745668..6746033 (-) Length   121 a.a.
NCBI ID   WP_014720320.1    Uniprot ID   A0A0J8G142
Organism   Pseudomonas fluorescens strain KF1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6740668..6751033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM720_RS30505 (IM720_30505) - 6743083..6745134 (-) 2052 WP_193689938.1 methyl-accepting chemotaxis protein -
  IM720_RS30510 (IM720_30510) - 6745131..6745655 (-) 525 WP_024077896.1 chemotaxis protein CheW -
  IM720_RS30515 (IM720_30515) pilH 6745668..6746033 (-) 366 WP_014720320.1 twitching motility response regulator PilH Machinery gene
  IM720_RS30520 (IM720_30520) pilG 6746080..6746487 (-) 408 WP_010564379.1 twitching motility response regulator PilG Regulator
  IM720_RS30525 (IM720_30525) gshB 6746727..6747680 (+) 954 WP_069077201.1 glutathione synthase -
  IM720_RS30530 (IM720_30530) - 6747758..6748663 (+) 906 WP_024077898.1 TonB family protein -
  IM720_RS30535 (IM720_30535) - 6748745..6749314 (+) 570 WP_069077200.1 YqgE/AlgH family protein -
  IM720_RS30540 (IM720_30540) ruvX 6749314..6749751 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  IM720_RS30545 (IM720_30545) pyrR 6749835..6750341 (+) 507 WP_024077900.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 121 a.a.        Molecular weight: 13227.23 Da        Isoelectric Point: 4.9105

>NTDB_id=437690 IM720_RS30515 WP_014720320.1 6745668..6746033(-) (pilH) [Pseudomonas fluorescens strain KF1]
MARVLIVDDSPTEMYKLTGMLEKHGHQVLKAENGADGVALARQEKPDAVLMDIVMPGLNGFQATRQLSKEPETNGIPIII
ITTKDQETDKIWGARQGAKDYLTKPVDEETLIATLNKVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=437690 IM720_RS30515 WP_014720320.1 6745668..6746033(-) (pilH) [Pseudomonas fluorescens strain KF1]
ATGGCACGCGTTCTGATCGTCGACGATTCGCCGACTGAAATGTACAAACTGACCGGTATGCTGGAAAAGCACGGCCACCA
GGTCCTCAAGGCCGAAAACGGCGCAGACGGTGTGGCCCTGGCTCGCCAGGAAAAGCCCGACGCGGTCCTGATGGACATCG
TGATGCCGGGCCTCAATGGTTTCCAGGCGACCCGCCAGCTGTCCAAGGAGCCGGAAACCAACGGTATTCCGATCATCATC
ATCACCACCAAGGACCAGGAGACCGACAAGATCTGGGGCGCGCGCCAAGGCGCCAAGGACTACCTGACCAAGCCGGTGGA
TGAAGAAACCCTGATCGCCACACTGAACAAGGTGTTGGCCGGCTGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J8G142

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364