Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IL987_RS13365 Genome accession   NZ_CP063214
Coordinates   2722607..2723284 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain ERP001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717607..2728284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL987_RS13330 gspG 2718087..2718524 (+) 438 WP_042092670.1 type II secretion system major pseudopilin GspG -
  IL987_RS13335 gspH 2718532..2719041 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  IL987_RS13340 gspI 2719038..2719415 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  IL987_RS13345 gspJ 2719408..2719995 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  IL987_RS13350 gspK 2719988..2720971 (+) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  IL987_RS13355 gspL 2720986..2722149 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  IL987_RS13360 gspM 2722146..2722607 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  IL987_RS13365 pilD 2722607..2723284 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  IL987_RS13370 bfr 2723313..2723789 (-) 477 WP_000675504.1 bacterioferritin -
  IL987_RS13375 bfd 2723861..2724055 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IL987_RS13380 chiA 2724224..2726926 (-) 2703 WP_193579139.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=437551 IL987_RS13365 WP_000178175.1 2722607..2723284(+) (pilD) [Escherichia coli strain ERP001]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=437551 IL987_RS13365 WP_000178175.1 2722607..2723284(+) (pilD) [Escherichia coli strain ERP001]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387