Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HF896_RS20745 Genome accession   NZ_CP051298
Coordinates   4321264..4322817 (-) Length   517 a.a.
NCBI ID   WP_013723188.1    Uniprot ID   A0A858ZZ03
Organism   Alicycliphilus denitrificans strain DP3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4316264..4327817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF896_RS20725 (HF896_20725) - 4317126..4317611 (-) 486 WP_013520823.1 putative toxin-antitoxin system toxin component, PIN family -
  HF896_RS20730 (HF896_20730) - 4317601..4318869 (-) 1269 WP_013520824.1 THUMP domain-containing protein -
  HF896_RS20735 (HF896_20735) - 4318982..4320805 (+) 1824 WP_013520825.1 methyl-accepting chemotaxis protein -
  HF896_RS20740 (HF896_20740) - 4320807..4321250 (-) 444 WP_013723187.1 thioesterase family protein -
  HF896_RS20745 (HF896_20745) rpoN/rpoN1 4321264..4322817 (-) 1554 WP_013723188.1 RNA polymerase factor sigma-54 Machinery gene
  HF896_RS20750 (HF896_20750) lptB 4322867..4323655 (-) 789 WP_168728198.1 LPS export ABC transporter ATP-binding protein -
  HF896_RS20755 (HF896_20755) lptA 4323664..4324308 (-) 645 WP_013520829.1 lipopolysaccharide transport periplasmic protein LptA -
  HF896_RS20760 (HF896_20760) - 4324428..4325084 (-) 657 WP_013520830.1 thiol:disulfide interchange protein DsbA/DsbL -
  HF896_RS20765 (HF896_20765) - 4325164..4325799 (-) 636 WP_013520831.1 SPOR domain-containing protein -
  HF896_RS20770 (HF896_20770) argS 4325812..4327515 (-) 1704 WP_013520832.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 517 a.a.        Molecular weight: 56290.29 Da        Isoelectric Point: 4.5584

>NTDB_id=437323 HF896_RS20745 WP_013723188.1 4321264..4322817(-) (rpoN/rpoN1) [Alicycliphilus denitrificans strain DP3]
MKPSLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTVDDAPLDEPVAPPSDEGGRRAEDATPSGAA
DADWTGDEGQNDAQMPDIAAPTDWDGDTDIPGDDREWGSDAPASVGSGSGDGDEADAADLAHGHESLADHLERQALALRL
SELDMAALRFLIGSLNDDGYLDEPLESLAASLADTGDPEQLDELVHRLTVALRLLQSLDPAGVGARGLAECLALQLQALD
REEEGDPEVIQAALCICRQPLDLLARRDVRRLAQACAASDDLTRAAMALIARLEPRPGRRFADVERNVIVPDVIVRKIGR
GAQQSFSVRLNPDVMPRLRVHDVYANALRGGSKGDGHQALQARLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFVHGE
LAMRPLVLREIADELGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRALIRQFVAAEDPHKPLSD
GQIADMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1554 bp        

>NTDB_id=437323 HF896_RS20745 WP_013723188.1 4321264..4322817(-) (rpoN/rpoN1) [Alicycliphilus denitrificans strain DP3]
ATGAAGCCCAGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCCATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAGGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCACGGTGGACGACG
CGCCGCTGGATGAGCCGGTCGCACCCCCGTCGGACGAGGGCGGCCGCCGGGCCGAGGATGCTACGCCTTCAGGAGCTGCT
GACGCAGATTGGACGGGCGATGAGGGCCAAAACGATGCTCAAATGCCCGATATCGCCGCTCCCACCGACTGGGACGGCGA
CACCGACATACCGGGCGACGACCGCGAGTGGGGCAGCGATGCGCCCGCAAGCGTCGGCAGCGGCTCTGGCGATGGCGACG
AGGCCGACGCCGCGGACCTGGCGCACGGCCACGAATCGCTCGCAGACCACCTTGAGCGCCAGGCCCTGGCGCTGCGCCTG
TCCGAGCTGGACATGGCGGCGCTGCGCTTTCTCATCGGCTCGCTCAACGACGACGGCTATCTGGACGAGCCGCTCGAATC
CCTGGCCGCGAGCCTGGCGGACACGGGCGACCCCGAGCAGCTCGATGAGCTGGTGCACCGCCTGACCGTGGCTCTGCGCC
TGCTGCAGAGCCTGGACCCCGCGGGCGTGGGCGCGCGCGGCCTGGCCGAATGCCTGGCGTTGCAGCTGCAGGCGCTGGAC
CGGGAGGAGGAGGGCGACCCCGAGGTGATCCAGGCCGCGCTGTGCATCTGCCGCCAGCCGCTCGACCTGCTGGCGCGGCG
CGACGTGCGCCGCCTGGCCCAGGCCTGCGCCGCGAGCGACGACCTCACGCGCGCCGCCATGGCGCTGATCGCGCGGCTGG
AGCCGCGCCCCGGGCGGCGCTTCGCCGACGTGGAGCGCAACGTCATCGTGCCCGACGTGATCGTCAGGAAGATCGGGCGC
GGCGCGCAGCAGAGCTTCAGCGTCCGCCTCAACCCCGACGTGATGCCGCGCCTGCGCGTGCACGACGTCTACGCCAACGC
CCTGCGCGGCGGCAGCAAGGGCGACGGCCACCAGGCGCTGCAGGCCAGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACA
TACAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAGCGCCATCGTGGAGCGGCAGAAGAACTTCTTCGTGCATGGCGAG
CTGGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTCGGCCTGCACGAATCCACCATCAGCCGCGTGACCAC
GGCCAAGTACATGGCCACGCCCCAGGGCACGTTCGAGCTCAAGTACTTCTTCGGCTCGGGCCTGGGCACCGAGACCGGCG
GCAGCGCGTCGAGCACGGCGGTGCGCGCGCTCATCCGGCAGTTCGTCGCCGCCGAGGATCCGCACAAGCCGCTGTCGGAC
GGCCAGATCGCCGACATGCTCAAGGAGCAGGGCATAGAGTGCGCGCGCCGCACCGTGGCCAAGTACCGAGAGGCGCTGAA
GATCGCGCCCGCCAACCTGCGCAAGGCGCTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-344)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(359-515)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858ZZ03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.805

100

0.528