Detailed information NTG0437294

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G8D25_RS15270
• Genome accession: NZ_CP051296
• Coordinates: 1580246..1581127 (-)
• Length: 293 a.a.
• NCBI ID: WP_014616067.1
• Uniprot ID: F6FY26
• Organism: Ralstonia solanacearum strain CIAT_078
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1575246..1586127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G8D25_RS15240 (G8D25_15235)	argJ	1575950..1577179 (+)	1230	WP_014616063.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
G8D25_RS15245 (G8D25_15240)	-	1577195..1578079 (+)	885	WP_014616064.1	ATP-binding protein	-
G8D25_RS15250 (G8D25_15245)	-	1578057..1578518 (+)	462	WP_014616065.1	NUDIX domain-containing protein	-
G8D25_RS15255 (G8D25_15250)	-	1578511..1578726 (-)	216	WP_003266032.1	DNA gyrase inhibitor YacG	-
G8D25_RS15260 (G8D25_15255)	zapD	1578743..1579501 (-)	759	WP_003266031.1	cell division protein ZapD	-
G8D25_RS15265 (G8D25_15260)	coaE	1579619..1580233 (-)	615	WP_014616066.1	dephospho-CoA kinase	-
G8D25_RS15270 (G8D25_15265)	pilD	1580246..1581127 (-)	882	WP_014616067.1	prepilin peptidase	Machinery gene
G8D25_RS15275 (G8D25_15270)	pilG	1581130..1582395 (-)	1266	WP_014616068.1	type II secretion system F family protein	Machinery gene
G8D25_RS15280 (G8D25_15275)	pilB	1582439..1584167 (-)	1729	Protein_1498	type IV-A pilus assembly ATPase PilB	-
G8D25_RS15285 (G8D25_15280)	-	1584414..1585691 (-)	1278	WP_014616070.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31552.49 Da
• Isoelectric Point: 6.8369

>NTDB_id=437294 G8D25_RS15270 WP_014616067.1 1580246..1581127(-) (pilD) [Ralstonia solanacearum strain CIAT_078]
MSGMFAIPTLALLPAWFVIGAAALLGLLVGSFLNVVIHRLPSMIEREEANYIAELRDEPLPHPEPYNLVVPRSACPSCGH
QIKAIENIPVISWLALRGRCSACKTPISWRYPAVELVTSVLTGACLWHFGPTWVAVASAVLLWFLIAGTMIDADTQLLPD
AVTQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWAIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLILLSS
VVGLVFGAARIARGAESDSPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=437294 G8D25_RS15270 WP_014616067.1 1580246..1581127(-) (pilD) [Ralstonia solanacearum strain CIAT_078]
ATGTCCGGGATGTTCGCCATTCCCACGCTGGCACTGCTGCCCGCGTGGTTCGTGATCGGCGCGGCGGCGCTGCTGGGGCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCTTGCCGAGCATGATCGAACGCGAGGAGGCCAACTACATCGCCG
AGCTGCGCGACGAGCCACTGCCGCATCCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGATCGAGAACATCCCGGTGATCAGTTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGACGCCCAT
CTCCTGGCGCTACCCCGCCGTGGAACTGGTGACCAGCGTACTGACCGGCGCCTGCCTGTGGCATTTCGGACCGACCTGGG
TGGCGGTGGCCTCGGCCGTGTTGCTGTGGTTCCTGATCGCCGGCACGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCGTCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCGACATGTTCGCGCGGCTGCCGGACGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGGCCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGCCGCGCTCGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGTTGCTGATCCTGCTGTCGTCG
GTGGTCGGCCTGGTGTTCGGGGCGGCGCGCATCGCGCGTGGCGCCGAAAGCGACTCACCGTTTTCGTTCGGCCCGTTCAT
CGCGGGCGCCGGCGTCATCGCCCTGCTGGCTGGCCCACAGCTGGTGGCATTGACGGGACTCGCACCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-247)

3D structure

• AlphaFold DB: F6FY26

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.585	90.444	0.485
pilD	Vibrio campbellii strain DS40M4	50.549	93.174	0.471
pilD	Neisseria gonorrhoeae MS11	49.627	91.468	0.454
pilD	Acinetobacter baumannii D1279779	46.992	90.785	0.427
pilD	Acinetobacter nosocomialis M2	46.617	90.785	0.423