Detailed information NTG0437291

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: G8D25_RS10315
• Genome accession: NZ_CP051296
• Coordinates: 523136..524269 (+)
• Length: 377 a.a.
• NCBI ID: WP_014618044.1
• Uniprot ID: F6G4B5
• Organism: Ralstonia solanacearum strain CIAT_078
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 518136..529269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G8D25_RS10290 (G8D25_10285)	-	518503..518751 (-)	249	WP_003262548.1	YfhL family 4Fe-4S dicluster ferredoxin	-
G8D25_RS10295 (G8D25_10290)	coaD	518802..519308 (-)	507	WP_003262550.1	pantetheine-phosphate adenylyltransferase	-
G8D25_RS10300 (G8D25_10295)	rsmD	519505..520161 (-)	657	WP_014618041.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
G8D25_RS10305 (G8D25_10300)	-	520239..521582 (-)	1344	WP_014618042.1	M16 family metallopeptidase	-
G8D25_RS10310 (G8D25_10305)	-	521582..523075 (-)	1494	WP_039550994.1	M16 family metallopeptidase	-
G8D25_RS10315 (G8D25_10310)	pilA	523136..524269 (+)	1134	WP_014618044.1	signal recognition particle-docking protein FtsY	Machinery gene
G8D25_RS10320 (G8D25_10315)	maiA	524474..525124 (-)	651	WP_014618045.1	maleylacetoacetate isomerase	-
G8D25_RS10325 (G8D25_10320)	-	525128..525829 (-)	702	WP_014618046.1	fumarylacetoacetate hydrolase family protein	-
G8D25_RS10330 (G8D25_10325)	-	525980..526606 (+)	627	WP_014618047.1	ANTAR domain-containing response regulator	-
G8D25_RS10335 (G8D25_10330)	-	526885..528147 (+)	1263	WP_014618048.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
G8D25_RS10340 (G8D25_10335)	ntrB	528197..529048 (+)	852	WP_042549711.1	nitrate ABC transporter permease	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 40168.48 Da
• Isoelectric Point: 5.8101

>NTDB_id=437291 G8D25_RS10315 WP_014618044.1 523136..524269(+) (pilA) [Ralstonia solanacearum strain CIAT_078]
MFSFWKKRKAEPQPAAEPAPPAAAPEAVQAPVPAPVASPTPAPAPAPVAVPDTPAAPAALDMQADDIETVPTPPVMEQAR
KGWMSRLRSGLSKTSKNLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLDELRRRVKAQRIETAESVKTALRDLLV
ELLHPLEKTMVLGRDQPMVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGQRNNVTVVAQES
GDPAAVIFDAVNAARARGIDIVMADTAGRLPTQMHLMEELKKVRRVIGKAMVTAPHETLLVIDANTGQNALAQVKAFDDA
LGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide

• Length: 1134 bp

>NTDB_id=437291 G8D25_RS10315 WP_014618044.1 523136..524269(+) (pilA) [Ralstonia solanacearum strain CIAT_078]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCAGAGCCCCAGCCGGCCGCCGAACCCGCGCCGCCCGCCGCCGCGCCCGAAGC
CGTCCAGGCCCCGGTACCGGCCCCGGTTGCCTCCCCCACCCCTGCCCCTGCACCCGCCCCGGTTGCCGTGCCGGATACGC
CGGCCGCGCCGGCTGCGCTGGACATGCAGGCCGACGACATCGAGACCGTACCGACGCCGCCCGTGATGGAGCAGGCGCGC
AAGGGCTGGATGTCTCGCCTGCGCTCGGGGCTGTCGAAGACCAGCAAGAACCTGACCACGCTGTTCGTCGGCGTGAAGGT
GGACGAGGCGCTGTTCGAGGAGCTGGAGACCGCGCTGCTGATGGCCGATGCCGGCGTGGATGCCACCGAATACCTGCTGG
ACGAGCTGCGCCGCCGCGTGAAGGCCCAGCGCATCGAGACCGCCGAGAGCGTGAAGACCGCGCTGCGCGACCTGCTCGTC
GAGCTGCTGCACCCGCTGGAAAAGACCATGGTGCTCGGCCGCGACCAGCCGATGGTGATCATGATCGCGGGCGTCAACGG
TGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGACCTACGGGCAGTCCGTGCTGCTGGCCGCCGGCG
ACACGTTCCGCGCCGCCGCCCGCGAGCAACTGGTGATCTGGGGCCAGCGCAACAACGTGACCGTGGTGGCGCAGGAGTCG
GGCGACCCGGCCGCCGTGATCTTCGACGCGGTGAACGCGGCGCGTGCCCGCGGCATCGACATTGTCATGGCCGACACCGC
CGGCCGCCTGCCGACCCAGATGCACCTGATGGAAGAGCTGAAGAAGGTGCGCCGCGTGATCGGCAAGGCCATGGTGACGG
CGCCGCACGAGACCCTGCTGGTGATCGACGCCAACACCGGCCAGAACGCGCTGGCGCAGGTGAAGGCTTTCGACGATGCA
CTCGGCCTGACCGGCCTGATCGTCACTAAGCTGGACGGCACCGCCAAAGGTGGCATCCTGGCCGCCATCGCGCGGCAGCG
GCCGGTGCCGGTGTATTTCATCGGCGTGGGCGAACAGGTCGAAGACCTGCAACCGTTCTCCGCGCGCGAATTCGCCGACG
CGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (177-377)

• SRP54-type protein, helical bundle domain (SRP54_N) (96-157)

3D structure

• AlphaFold DB: F6G4B5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.202	100	0.565