NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	IMY14_RS14800	Genome accession	NZ_CP063150
Coordinates	2813665..2814669 (-)	Length	334 a.a.
NCBI ID	WP_003229718.1	Uniprot ID	O32055
Organism	Bacillus subtilis strain s-16
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2808665..2819669

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMY14_RS14770	yrbG	2809639..2810295 (-)	657	WP_015483509.1	DUF421 domain-containing protein	-
IMY14_RS14775	-	2810493..2810882 (+)	390	WP_031315243.1	TIGR04086 family membrane protein	-
IMY14_RS14780	yajC	2810939..2811205 (-)	267	WP_004398708.1	preprotein translocase subunit YajC	-
IMY14_RS14785	tgt	2811242..2812387 (-)	1146	WP_003229725.1	tRNA guanosine(34) transglycosylase Tgt	-
IMY14_RS14790	queA	2812414..2813442 (-)	1029	WP_174616771.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
IMY14_RS14795	yrzS	2813472..2813672 (-)	201	WP_003222669.1	DUF2905 domain-containing protein	-
IMY14_RS14800	ruvB	2813665..2814669 (-)	1005	WP_003229718.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
IMY14_RS14805	ruvA	2814680..2815285 (-)	606	WP_003229717.1	Holliday junction branch migration protein RuvA	Machinery gene
IMY14_RS14810	bofC	2815424..2815936 (-)	513	WP_047182964.1	sporulation cell-cell signaling protein BofC	-
IMY14_RS14815	csbX	2815984..2817291 (-)	1308	WP_003246197.1	MFS transporter	-
IMY14_RS14820	iolG	2817362..2818387 (-)	1026	WP_174616772.1	inositol 2-dehydrogenase	-
IMY14_RS14825	yrzF	2818625..2819272 (+)	648	WP_047182966.1	phosphotransferase	-
IMY14_RS14830	-	2819318..2819440 (-)	123	WP_003229707.1	hypothetical protein	-
IMY14_RS22010	-	2819525..2819599 (+)	75	Protein_2910	hypothetical protein	-

Sequence

Protein

Download Length: 334 a.a. Molecular weight: 37467.78 Da Isoelectric Point: 5.1631

                                >NTDB_id=437134 IMY14_RS14800 WP_003229718.1 2813665..2814669(-) (ruvB) [Bacillus subtilis strain s-16]

MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE

LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT

RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI

TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP

AVYHHFQMEAPRYD

Nucleotide

Download Length: 1005 bp

                                >NTDB_id=437134 IMY14_RS14800 WP_003229718.1 2813665..2814669(-) (ruvB) [Bacillus subtilis strain s-16]

ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC

ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG

ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCCATTGTAGCCAATGAAATGGGAGTGGAA

TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT

ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG

ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG

AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTGATGTCTCGCCTTGAGTACTATACACAAGAAGAACT

GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT

CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT

ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT

GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA

CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG

GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae R6	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae D39	60.429	97.605	0.59
ruvB	Synechocystis sp. PCC 6803	57.143	98.503	0.563
ruvB	Helicobacter pylori 26695	52.744	98.204	0.518

Detailed information NTG0437134