Detailed information NTG0436946

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HGD77_RS08230
• Genome accession: NZ_CP051208
• Coordinates: 1731513..1732712 (-)
• Length: 399 a.a.
• NCBI ID: WP_004731151.1
• Uniprot ID: A0A4V1VP90
• Organism: Acinetobacter sp. NEB149
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1726513..1737712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HGD77_RS08215 (HGD77_08220)	-	1728538..1729566 (+)	1029	WP_004731146.1	AraC family transcriptional regulator	-
HGD77_RS08220 (HGD77_08225)	gcvH	1729649..1730023 (+)	375	WP_004731147.1	glycine cleavage system protein GcvH	-
HGD77_RS08225 (HGD77_08230)	-	1730262..1731437 (+)	1176	WP_004731149.1	MFS transporter	-
HGD77_RS08230 (HGD77_08235)	pilU	1731513..1732712 (-)	1200	WP_004731151.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HGD77_RS08235 (HGD77_08240)	-	1733291..1733719 (-)	429	WP_000877730.1	hypothetical protein	-
HGD77_RS08240 (HGD77_08245)	-	1733915..1735249 (+)	1335	WP_138743423.1	ISNCY family transposase	-
HGD77_RS08245 (HGD77_08250)	-	1735868..1736917 (-)	1050	WP_004731158.1	3-deoxy-7-phosphoheptulonate synthase	-

Sequence

Protein

• Length: 399 a.a.
• Molecular weight: 45064.53 Da
• Isoelectric Point: 6.3929

>NTDB_id=436946 HGD77_RS08230 WP_004731151.1 1731513..1732712(-) (pilU) [Acinetobacter sp. NEB149]
MFTAELLDEAEKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAINVPGVSRFRVNVFVQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGAISYEEALRNADSTNELRLQIKLKSNRQDSTAPASEATQFTMMQDPKADEKEAENTSVSE

Nucleotide

• Length: 1200 bp

>NTDB_id=436946 HGD77_RS08230 WP_004731151.1 1731513..1732712(-) (pilU) [Acinetobacter sp. NEB149]
ATGTTCACGGCAGAATTGTTGGACGAAGCAGAAAAATTCATGCATCACATGCTGACCAAGGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCGAGTATTAAACATCAAGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CAGCTGAAAAAACCAAGCTGTTTGCCTACAGCCTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTTGAGTGC
AATTTTGCCATCAATGTGCCGGGCGTTTCCCGTTTCCGGGTAAATGTGTTTGTGCAACAACTTAATGTCGGTATGGTAAT
TCGTACCATTACTGCAGAAATTCCAAATTTCCAGAAGCTGAAATTACCGGAATCGCTGAAACATGTGATTATGGAAAAAC
GTGGACTGGTACTGGTAGTCGGAGGAACGGGTTCAGGTAAATCAACTTCACTCGCAGCCATGATTGACTACCGAAATGAA
AATTCAGCAGGGCATATCATTACCGTAGAAGATCCGGTTGAATATGTGCATAAACATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCTTGGCATAATGCACTGAAAAATACCTTGCGTCAGGCTCCGGATGTAATTCTGATCG
GGGAAATTCGCGATACTGAAACTATGGAACATGCGATTGCTTTTGCGGAAACTGGCCATTTGTGTTTGGGAACTCTGCAT
TCCAATAATGCCAACCAGACTTTGGATCGGATTATCAACTTCTTCCCTGAAGAACGCCGGAATCAGTTATTGATGGATTT
GTCTTCAAATATGAAGGCAATTATTTCACAGCGTCTGATTCGGACTCAAGATGGTAAAGGCCGTCGGGCAGCGATTGAAA
TCATGCTGAATACGCCTTTGATTTCCGACCTGATCCTGAAAGGCGAGTTTCATGAGCTGAAAGCCATTATGGCTAAATCC
CGTGAACTGGGCATGCAGACTTTTGACCAAGCCTTATTTGATCTGTATAACCAAGGCGCCATTTCCTATGAAGAAGCTTT
GCGTAATGCCGATTCTACCAATGAGTTACGCCTGCAGATCAAGCTGAAAAGTAATCGACAGGACAGTACTGCACCAGCTA
GTGAAGCAACCCAATTCACCATGATGCAAGATCCAAAAGCGGATGAGAAAGAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

3D structure

• AlphaFold DB: A0A4V1VP90

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.207	87.218	0.534
pilU	Acinetobacter baylyi ADP1	56.196	86.967	0.489
pilU	Vibrio cholerae strain A1552	51.913	91.729	0.476
pilT	Pseudomonas aeruginosa PAK	44.479	81.704	0.363
pilT	Acinetobacter nosocomialis M2	43.636	82.707	0.361
pilT	Acinetobacter baumannii D1279779	43.636	82.707	0.361
pilT	Acinetobacter baumannii strain A118	43.636	82.707	0.361