Detailed information    

experimental Experimentally validated

Overview

Name   rec2   Type   Machinery gene
Locus tag   HI_0061 Genome accession   L42023
Coordinates   63196..65562 (-) Length   788 a.a.
NCBI ID   AAC21739.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

Two mutations, rec2 and comF, were not transformable despite taking up near-normal amounts of DNA. Rec2 homologs are needed for the transport of a single DNA strand across the cytoplasmic membrane in all species.


Genomic Context

Location: 58196..70562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0058 - 59486..60250 (-) 765 AAC21736.1 3-deoxy-D-manno-octulosonate cytidylyltransferase (kdsB) -
  HI_0059 - 60321..61319 (-) 999 AAC21737.1 conserved hypothetical protein -
  HI_0060 - 61392..63155 (-) 1764 AAC21738.1 ABC transporter, ATP-binding protein (msbA) -
  HI_0061 rec2 63196..65562 (-) 2367 AAC21739.1 recombination protein (rec2) Machinery gene
  HI_0062 - 65821..66258 (+) 438 AAC21740.1 dnaK suppressor protein (dksA) -
  HI_0063 - 66506..67864 (+) 1359 AAC21741.1 poly(A) polymerase (pcnB) -
  HI_0064 - 67873..68355 (+) 483 AAC21742.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine-pyrophosphokinase (folK) -
  HI_0065 - 68431..68907 (+) 477 AAC21743.1 conserved hypothetical protein -
  HI_0066 - 68915..70213 (+) 1299 AAC21744.1 N-acetylmuramoyl-L-alanine amidase, putative -

Sequence

Protein


Download         Length: 788 a.a.        Molecular weight: 89355.73 Da        Isoelectric Point: 9.9449

>NTDB_id=1359 HI_0061 AAC21739.1 63196..65562(-) (rec2) [Haemophilus influenzae Rd KW20]
MKLNLITLVVLLIVADLTLLFLPQPLLLPWQVALVIALVLIFLFIFLRRNFLVSLAFFVASLGYFHYSALSLSQQAQNIT
AQKQVVTFKIQEILHQQDYQTLIATATLENNLQEQRIFLNWKAKEVPQLSEIWQAEISLRSLSARLNFGGFDRQQWYFSK
GITAVGTVKSAVKIADVSSLRAEKLQQVKKQTEGLSLQGLLIALAFGERAWLDKTTWSIYQQTNTAHLIAISGLHIGLAM
GIGFCLARVVQVFFPTRFIHPYFPLVFGVLFALIYAYLAGFSVPTFRAISALVFVLFIQIMRRHYSPIQFFTLVVGFLLF
CDPLMPLSVSFWLSCGAVGCLLLWYRYVPFSLFQWKNRPFSPKVRWIFSLFHLQFGLLLFFTPLQLFLFNGLSLSGFLAN
FMAVPIYSFLLVPLILFAVFTNGTMFSWQLANKLAEGITGLISVFQGNWLTVSFNLALGLTALCAGIFMLIIWNIYREPE
ISSSNWQIKRAKFFTLNLSKPLLKNERINVLRCSFGIILLCFTILLFKQLSKPTWQVDTLDVGQGLATLIVKNGKGILYD
TGSSWRGGSMAELEILPYLQREGIVLEKLILSHDDNDHAGGASTILKAYPNVELITPSRKNYGENYRTFCTAGRDWHWQG
LHFQILSPHNVVTRADNSHSCVILVDDGKNSVLLTGDAEAKNEQIFARTLGKIDVLQVGHHGSKTSTSEYLLSQVRPDVA
IISSGRWNPWKFPHYSVMERLHRYKSAVENTAVSGQVRVNFFQDRLEIQQARTKFSPWYARVIGLSKE

Nucleotide


Download         Length: 2367 bp        

>NTDB_id=1359 HI_0061 AAC21739.1 63196..65562(-) (rec2) [Haemophilus influenzae Rd KW20]
ATGAAATTAAACTTAATAACTTTAGTTGTCTTGTTAATTGTCGCGGATTTAACGTTGTTATTTCTACCGCAACCGTTGCT
ATTGCCTTGGCAAGTTGCTCTCGTTATTGCGCTTGTTTTGATTTTTCTTTTTATTTTCTTGCGTAGAAATTTCTTAGTTA
GCCTTGCTTTTTTTGTTGCCTCTCTTGGCTATTTTCATTATTCGGCTTTGAGTTTATCACAACAAGCTCAAAATATTACC
GCTCAAAAGCAAGTGGTAACTTTTAAGATTCAAGAAATTTTGCACCAACAGGATTATCAAACGCTTATCGCCACAGCAAC
ATTGGAGAATAATTTGCAAGAACAACGAATTTTCTTAAATTGGAAAGCGAAAGAGGTGCCTCAATTATCGGAAATTTGGC
AAGCTGAAATTTCTTTACGTTCCCTTTCTGCACGATTAAATTTCGGTGGGTTTGATCGGCAACAATGGTATTTTTCAAAA
GGAATTACGGCTGTTGGAACGGTAAAAAGTGCGGTGAAAATTGCGGATGTTTCATCATTGCGTGCAGAAAAATTGCAACA
AGTAAAGAAGCAAACGGAAGGATTATCTCTACAAGGTTTATTGATTGCCTTAGCTTTTGGCGAACGGGCTTGGTTAGATA
AAACCACTTGGTCAATTTACCAACAAACCAATACCGCACATCTTATTGCTATTTCTGGCTTACATATTGGGTTGGCTATG
GGAATTGGATTTTGCTTGGCGCGTGTTGTGCAAGTGTTCTTTCCTACGCGTTTTATTCATCCTTATTTTCCTTTAGTTTT
TGGTGTTTTATTTGCTTTAATTTATGCGTATTTGGCTGGTTTTAGTGTGCCAACTTTTCGTGCCATTTCAGCACTTGTTT
TCGTTTTATTTATTCAAATAATGAGGCGACATTATTCGCCCATTCAGTTTTTTACGTTGGTTGTCGGATTCTTGCTTTTC
TGCGATCCATTAATGCCGCTTTCGGTCAGTTTTTGGCTTTCTTGTGGAGCGGTTGGGTGTTTGCTCCTCTGGTATCGTTA
TGTGCCTTTTTCACTTTTTCAATGGAAAAATCGCCCTTTTTCTCCAAAAGTGCGGTGGATTTTTAGTTTATTTCATTTGC
AATTTGGGTTATTGCTCTTTTTTACACCTTTGCAACTTTTTCTATTTAATGGCTTATCGTTGAGTGGATTTTTAGCCAAT
TTTATGGCGGTTCCAATTTATAGTTTTTTGCTTGTGCCATTAATTTTATTTGCCGTTTTTACTAACGGCACAATGTTTTC
TTGGCAACTAGCAAACAAGCTAGCCGAAGGAATTACTGGGTTAATTTCTGTTTTTCAAGGGAATTGGCTCACGGTTTCAT
TTAATTTAGCATTGGGTTTAACCGCACTTTGTGCAGGAATTTTTATGTTAATTATTTGGAATATTTACCGAGAACCCGAG
ATTTCATCATCAAACTGGCAAATAAAACGAGCAAAATTTTTTACATTAAATCTCAGTAAGCCTTTGCTAAAAAATGAACG
AATCAACGTTTTGCGATGTTCTTTCGGCATTATCTTACTGTGTTTTACGATTTTGTTGTTTAAACAATTGAGCAAGCCAA
CTTGGCAGGTAGATACTTTAGATGTGGGGCAGGGGTTAGCTACGTTGATTGTGAAAAATGGTAAAGGGATTCTTTATGAT
ACGGGTTCTTCTTGGCGAGGTGGAAGTATGGCTGAGTTGGAAATTTTGCCTTATTTACAAAGAGAAGGGATTGTTTTGGA
AAAATTGATTTTAAGCCACGACGATAACGATCACGCAGGTGGTGCTTCGACAATTTTAAAGGCGTATCCCAATGTGGAAT
TGATTACCCCTTCACGGAAAAACTATGGGGAAAATTACCGCACTTTTTGTACTGCTGGGCGTGATTGGCATTGGCAAGGG
TTGCATTTTCAAATACTTTCTCCTCACAACGTTGTGACACGAGCTGATAATTCCCATTCTTGTGTGATTTTAGTCGATGA
TGGAAAGAATAGCGTTTTGCTAACTGGCGATGCTGAAGCAAAAAATGAGCAAATTTTTGCCCGCACTTTAGGCAAAATCG
ATGTGTTGCAAGTGGGGCATCATGGGAGTAAAACATCGACAAGTGAATACTTGCTTTCTCAGGTTAGACCAGATGTAGCG
ATTATTTCTAGTGGGCGTTGGAATCCGTGGAAATTCCCTCATTATTCGGTTATGGAAAGGCTTCATCGCTATAAAAGTGC
GGTAGAAAATACCGCTGTTTCGGGGCAAGTGCGGGTAAATTTTTTTCAAGACCGATTAGAAATCCAGCAAGCTCGCACAA
AATTTTCCCCTTGGTATGCGCGTGTAATTGGATTATCAAAGGAATAA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(541-725)

Competence protein (Competence)

(204-473)

Domain of unknown function (DUF4131) (DUF4131)

(28-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]