Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HFV01_RS18135 Genome accession   NZ_CP051185
Coordinates   3859848..3861860 (-) Length   670 a.a.
NCBI ID   WP_187758012.1    Uniprot ID   -
Organism   Limnospira fusiformis SAG 85.79     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3854848..3866860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFV01_RS18110 (HFV01_18610) - 3854976..3855917 (-) 942 WP_006620584.1 alpha/beta fold hydrolase -
  HFV01_RS18115 (HFV01_18615) - 3855977..3856600 (-) 624 WP_006620585.1 hypothetical protein -
  HFV01_RS18120 (HFV01_18620) - 3856675..3857793 (-) 1119 WP_008049503.1 type II secretion system F family protein -
  HFV01_RS18125 (HFV01_18625) - 3857985..3859088 (-) 1104 WP_006620587.1 type IV pilus twitching motility protein PilT -
  HFV01_RS18130 (HFV01_18630) - 3859206..3859586 (-) 381 Protein_3677 zinc ribbon domain-containing protein -
  HFV01_RS18135 (HFV01_18635) pilB/pilB1 3859848..3861860 (-) 2013 WP_187758012.1 GspE/PulE family protein Machinery gene
  HFV01_RS18140 (HFV01_18640) grpE 3862449..3863210 (+) 762 WP_006620592.1 nucleotide exchange factor GrpE -
  HFV01_RS18145 (HFV01_18645) dnaK 3863374..3865350 (+) 1977 WP_006620593.1 molecular chaperone DnaK -
  HFV01_RS18150 (HFV01_18650) dnaJ 3865535..3866665 (+) 1131 WP_006620594.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75163.02 Da        Isoelectric Point: 5.0014

>NTDB_id=436716 HFV01_RS18135 WP_187758012.1 3859848..3861860(-) (pilB/pilB1) [Limnospira fusiformis SAG 85.79]
MTNTSSPRRTALVAQSATPFGNKLIQSGYIDPERLQQAIQDSRGGKPLTEVLESITGQPLPPELARHYKKQQLFELKIVY
GVEAFDLEIDQISVAELEEIIKKLETSIETCKRHRFVPWKIIESEPTPSVMMAMVDPDNLSALDELRNQILRPVGWKLQR
RVITQEDYDKLITAYGEEEVKRKQVEAAREEERKLQFGEGEFEDVELVEASEDMGDMNLDAQLQEAETAPVIKAVNVILA
RALSEGVSDIHVEPQEHDMRIRMRKDGVLQEYFSFPKKVISAITSRFKIIADLDIAERRQAQDGRIRRIYQGRTVDFRVN
SLPSRYGEKIVLRILDSSSTQLGLDKLISDQETLEMVRETASRPFGLILVTGPTGSGKSTSLYSVLAERNDPGINISTAE
DPIEYALPGITQCQVIREKDLTFANILRAFLRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDE
MGVEPFMVSAALLGILAQRLMRRVCTECRIPYTPTPQEFGRYGLSASGEVDITFYKANTVPADQRRGLADRDELCKKCGG
LGYKGRVGVYEFLKVTERMQILITKGAPTEQIKEAAVEEGMKTLLAYSLQLVREGHTTFEEVERVTFTDSALESELKAKR
KQGLTCKGCAAELKPEWLDCPYCLTPRFTM

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=436716 HFV01_RS18135 WP_187758012.1 3859848..3861860(-) (pilB/pilB1) [Limnospira fusiformis SAG 85.79]
ATGACCAATACATCATCCCCCCGGCGAACTGCTCTTGTTGCTCAAAGTGCAACTCCCTTTGGTAATAAACTTATTCAGTC
AGGATATATTGACCCTGAGCGACTACAGCAGGCTATTCAGGACTCTCGTGGCGGAAAACCTCTCACCGAAGTTCTCGAAT
CCATTACCGGTCAGCCTCTACCCCCGGAATTAGCCCGTCACTATAAAAAACAACAGCTATTTGAGTTAAAAATTGTCTAT
GGGGTTGAGGCCTTTGACCTAGAAATAGATCAGATTTCGGTGGCTGAACTAGAAGAAATCATCAAAAAGCTGGAAACTTC
TATTGAGACCTGCAAACGACATCGTTTTGTGCCTTGGAAAATCATCGAGTCGGAGCCAACTCCTAGCGTGATGATGGCAA
TGGTTGACCCAGATAACCTGAGTGCTTTGGATGAGTTGAGAAACCAAATCCTGCGTCCGGTAGGATGGAAACTACAGCGG
CGGGTTATTACTCAAGAAGATTATGACAAACTGATTACAGCCTACGGTGAGGAGGAAGTTAAGCGCAAGCAAGTAGAAGC
GGCTCGGGAGGAAGAGAGAAAACTCCAGTTTGGCGAGGGTGAGTTTGAGGATGTAGAACTGGTAGAAGCCTCTGAGGACA
TGGGAGACATGAACCTCGATGCACAGCTCCAAGAGGCGGAAACTGCACCAGTTATTAAGGCTGTGAATGTGATTCTGGCG
AGAGCTCTGTCCGAGGGTGTTTCTGATATCCATGTGGAACCCCAAGAGCATGATATGCGGATTCGGATGCGTAAAGATGG
GGTTTTGCAGGAATATTTTAGTTTCCCGAAAAAGGTGATTTCTGCTATTACCTCCCGCTTTAAAATTATTGCCGATCTTG
ATATCGCTGAACGTCGCCAAGCTCAGGATGGCCGGATTCGTCGGATTTATCAGGGCCGGACGGTGGATTTTCGGGTCAAT
AGCCTGCCGAGTCGCTATGGGGAAAAGATTGTTCTGCGGATTCTTGATAGTTCTTCGACTCAATTGGGTTTGGATAAGTT
GATCTCTGACCAGGAAACTTTGGAAATGGTCCGAGAAACTGCTAGTCGTCCTTTTGGGTTGATTTTGGTAACAGGACCTA
CGGGTTCTGGTAAATCTACATCCTTGTATTCGGTTTTGGCAGAGCGCAATGATCCGGGTATTAATATTAGTACGGCGGAA
GACCCGATTGAGTATGCTCTACCTGGTATTACCCAATGTCAGGTGATTCGGGAAAAAGATTTGACTTTTGCTAATATCCT
GCGGGCATTCTTACGACAAGACCCTGATGTTTTGCTAGTGGGGGAAACGCGGGACTTGGAAACGGCGAAAACTGCTATTG
AAGCGGCTCTGACGGGACACTTGGTTTTAACTACTCTTCACACTAATGATGCGGCGGGGGCGATCGCTCGTTTGGATGAG
ATGGGAGTTGAACCTTTCATGGTCTCTGCCGCTCTGTTGGGTATCCTGGCTCAACGTTTGATGCGGCGGGTATGTACTGA
ATGTCGTATACCCTATACCCCAACTCCCCAAGAATTTGGTCGTTATGGTCTTTCTGCTTCTGGGGAGGTGGATATTACTT
TCTATAAGGCAAATACTGTTCCGGCAGATCAACGCCGGGGACTAGCCGATCGCGACGAGTTATGTAAAAAATGTGGTGGT
CTAGGCTATAAGGGCCGGGTTGGGGTTTATGAATTCCTTAAAGTAACCGAGCGGATGCAAATTCTGATTACTAAGGGAGC
GCCCACTGAACAAATTAAGGAGGCTGCTGTTGAGGAAGGGATGAAAACTCTCTTAGCCTACAGTTTGCAGTTGGTGCGTG
AAGGCCATACAACTTTTGAGGAGGTGGAACGGGTAACCTTTACAGATTCGGCTCTGGAATCGGAGTTGAAGGCTAAACGG
AAGCAGGGTTTGACCTGTAAAGGATGTGCGGCGGAATTAAAGCCGGAATGGTTGGACTGTCCCTATTGTTTGACTCCTCG
GTTTACTATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(238-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.805

99.254

0.613