Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HF906_RS14725 Genome accession   NZ_CP051175
Coordinates   3157974..3159473 (+) Length   499 a.a.
NCBI ID   WP_003269865.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain Rs5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152974..3164473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF906_RS14700 (HF906_14740) - 3154153..3155136 (+) 984 WP_003269858.1 KpsF/GutQ family sugar-phosphate isomerase -
  HF906_RS14705 (HF906_14745) - 3155169..3155756 (+) 588 WP_141515174.1 KdsC family phosphatase -
  HF906_RS14710 (HF906_14750) lptC 3155758..3156390 (+) 633 WP_003269861.1 LPS export ABC transporter periplasmic protein LptC -
  HF906_RS14715 (HF906_14755) lptA 3156458..3157054 (+) 597 WP_003269863.1 lipopolysaccharide transport periplasmic protein LptA -
  HF906_RS14720 (HF906_14760) lptB 3157088..3157876 (+) 789 WP_003262277.1 LPS export ABC transporter ATP-binding protein -
  HF906_RS14725 (HF906_14765) rpoN/rpoN1 3157974..3159473 (+) 1500 WP_003269865.1 RNA polymerase factor sigma-54 Machinery gene
  HF906_RS14730 (HF906_14770) hpf 3159525..3159878 (+) 354 WP_003269867.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HF906_RS14735 (HF906_14775) ptsN 3160228..3160683 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HF906_RS14740 (HF906_14780) hprK 3160754..3161728 (+) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  HF906_RS14745 (HF906_14785) - 3161919..3162266 (+) 348 WP_003269871.1 PsiF family protein -
  HF906_RS14750 (HF906_14790) rapZ 3162360..3163253 (+) 894 WP_003269873.1 RNase adapter RapZ -
  HF906_RS14755 (HF906_14795) - 3163331..3163984 (+) 654 WP_003269875.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55214.60 Da        Isoelectric Point: 4.8496

>NTDB_id=436612 HF906_RS14725 WP_003269865.1 3157974..3159473(+) (rpoN/rpoN1) [Ralstonia solanacearum strain Rs5]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPLPTE
SPPPAANGSDHTDTAEAPYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSTR
DKGLVIFLIESLDDEGYLTGSLDEILTDLPAELEIELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=436612 HF906_RS14725 WP_003269865.1 3157974..3159473(+) (rpoN/rpoN1) [Ralstonia solanacearum strain Rs5]
ATGAAACAGTCGCTCCAGCTCCGCCTCTCCCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGGGCCGATGGCTCGGTCAGCGCACTGCAGAGCAACAGCGCCACCCCGCTGCCGACCGAG
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACACCGACACGGCCGAGGCACCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACGATCGCGCTC
CGATGCAGTTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCACGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAGGGCTACCTGACCGGCTCGCTCGACGAGATCCTCAC
CGACCTGCCGGCCGAACTCGAGATCGAACTCGACGAACTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAATTGCTCGCCGCGCGCGACTACACGCGTCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCGGACGTGATGCCACGCCTG
CGCATCAACGACATGTACGCCCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.194

100

0.972